Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31609 | 3' | -54.5 | NC_006879.1 | + | 22684 | 0.66 | 0.602265 |
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Target: 5'- gCCUGGGAGAgcGCGGcgacGUCca--AGGGCa -3' miRNA: 3'- -GGACCCUCUa-CGUC----UAGacacUCCCG- -5' |
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| 31609 | 3' | -54.5 | NC_006879.1 | + | 33926 | 0.67 | 0.579535 |
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Target: 5'- aUCUGGGAcGUuuuuuuucuguaGCGGAUaugaaUGUGAGGGg -3' miRNA: 3'- -GGACCCUcUA------------CGUCUAg----ACACUCCCg -5' |
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| 31609 | 3' | -54.5 | NC_006879.1 | + | 1694 | 0.67 | 0.550278 |
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Target: 5'- uCgUGGGAGuacAUGCuGGAuuacauggcgauggcUCUGUgGAGGGCc -3' miRNA: 3'- -GgACCCUC---UACG-UCU---------------AGACA-CUCCCG- -5' |
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| 31609 | 3' | -54.5 | NC_006879.1 | + | 24252 | 0.69 | 0.449343 |
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Target: 5'- cUCUGGGuGAUgacGCAGG-CcGUGAGGGg -3' miRNA: 3'- -GGACCCuCUA---CGUCUaGaCACUCCCg -5' |
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| 31609 | 3' | -54.5 | NC_006879.1 | + | 8062 | 0.69 | 0.419299 |
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Target: 5'- gCCgGGGGGuagagGCGGcUCUGcgccgGGGGGCa -3' miRNA: 3'- -GGaCCCUCua---CGUCuAGACa----CUCCCG- -5' |
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| 31609 | 3' | -54.5 | NC_006879.1 | + | 24057 | 0.7 | 0.389568 |
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Target: 5'- aCCUGGGuuucgcuGAguagcucgguguUGCAGGccagcgaUCUGUGGGGGg -3' miRNA: 3'- -GGACCCu------CU------------ACGUCU-------AGACACUCCCg -5' |
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| 31609 | 3' | -54.5 | NC_006879.1 | + | 14542 | 0.72 | 0.296533 |
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Target: 5'- gCUGcGcAGcgGCAGGguuccgugaUCUGUGAGGGCg -3' miRNA: 3'- gGAC-CcUCuaCGUCU---------AGACACUCCCG- -5' |
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| 31609 | 3' | -54.5 | NC_006879.1 | + | 25421 | 0.72 | 0.288918 |
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Target: 5'- -gUGGGcucGGAUGCcguuaacucgugAGAUCaUGUGGGGGCc -3' miRNA: 3'- ggACCC---UCUACG------------UCUAG-ACACUCCCG- -5' |
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| 31609 | 3' | -54.5 | NC_006879.1 | + | 4336 | 1.14 | 0.000268 |
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Target: 5'- gCCUGGGAGAUGCAGAUCUGUGAGGGCa -3' miRNA: 3'- -GGACCCUCUACGUCUAGACACUCCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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