Results 1 - 14 of 14 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31609 | 5' | -54.5 | NC_006879.1 | + | 25244 | 0.66 | 0.638303 |
Target: 5'- aAGGagauucccaccCCCuacauGUGGAGCUAUCAgCCCCa -3' miRNA: 3'- -UCC-----------GGGuc---UACCUUGAUAGUaGGGGg -5' |
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31609 | 5' | -54.5 | NC_006879.1 | + | 24780 | 0.66 | 0.626827 |
Target: 5'- uGGGCCCGccaGGAGCaAUa--CCCCCg -3' miRNA: 3'- -UCCGGGUcuaCCUUGaUAguaGGGGG- -5' |
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31609 | 5' | -54.5 | NC_006879.1 | + | 8555 | 0.66 | 0.615356 |
Target: 5'- uGGCCCAGGUGGucauGCgagCGcgcgacUCCUUCg -3' miRNA: 3'- uCCGGGUCUACCu---UGauaGU------AGGGGG- -5' |
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31609 | 5' | -54.5 | NC_006879.1 | + | 4905 | 0.66 | 0.603901 |
Target: 5'- aAGcGCCCAGcccGGGGC--UCAUCCCUUc -3' miRNA: 3'- -UC-CGGGUCua-CCUUGauAGUAGGGGG- -5' |
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31609 | 5' | -54.5 | NC_006879.1 | + | 12377 | 0.67 | 0.547216 |
Target: 5'- cGGCUCGGAacaUGGAACcGUCcuuuuacUCCgCCCa -3' miRNA: 3'- uCCGGGUCU---ACCUUGaUAGu------AGG-GGG- -5' |
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31609 | 5' | -54.5 | NC_006879.1 | + | 28415 | 0.67 | 0.547216 |
Target: 5'- cGGGCCUguAGAgGGGGCUAUCcUCUgUCa -3' miRNA: 3'- -UCCGGG--UCUaCCUUGAUAGuAGGgGG- -5' |
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31609 | 5' | -54.5 | NC_006879.1 | + | 9966 | 0.67 | 0.536061 |
Target: 5'- cGuGCCCAccAUGGAGCgcuUUcgCCCCCu -3' miRNA: 3'- uC-CGGGUc-UACCUUGau-AGuaGGGGG- -5' |
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31609 | 5' | -54.5 | NC_006879.1 | + | 19948 | 0.69 | 0.450297 |
Target: 5'- cAGGCCUAcccGGcAACUAUCccuAUCCCCUg -3' miRNA: 3'- -UCCGGGUcuaCC-UUGAUAG---UAGGGGG- -5' |
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31609 | 5' | -54.5 | NC_006879.1 | + | 4372 | 0.69 | 0.430037 |
Target: 5'- --cCCCAGGUGG-AC-AUgAUCCCCCc -3' miRNA: 3'- uccGGGUCUACCuUGaUAgUAGGGGG- -5' |
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31609 | 5' | -54.5 | NC_006879.1 | + | 16294 | 0.7 | 0.400678 |
Target: 5'- cGGGUUgGGaAUGGuAACUGUCuUUCCCCg -3' miRNA: 3'- -UCCGGgUC-UACC-UUGAUAGuAGGGGG- -5' |
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31609 | 5' | -54.5 | NC_006879.1 | + | 7683 | 0.73 | 0.25322 |
Target: 5'- gGGGCUU-GGUGGGGCgg-CAUCCCCUu -3' miRNA: 3'- -UCCGGGuCUACCUUGauaGUAGGGGG- -5' |
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31609 | 5' | -54.5 | NC_006879.1 | + | 10310 | 0.76 | 0.162429 |
Target: 5'- uGGCCCGGcugGGAGCgc-CGUCCCCa -3' miRNA: 3'- uCCGGGUCua-CCUUGauaGUAGGGGg -5' |
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31609 | 5' | -54.5 | NC_006879.1 | + | 7188 | 0.76 | 0.162429 |
Target: 5'- uGGGCCCGGgcGGAGCUggGUC-UCUCCUc -3' miRNA: 3'- -UCCGGGUCuaCCUUGA--UAGuAGGGGG- -5' |
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31609 | 5' | -54.5 | NC_006879.1 | + | 4299 | 1.13 | 0.000335 |
Target: 5'- cAGGCCCAGAUGGAACUAUCAUCCCCCa -3' miRNA: 3'- -UCCGGGUCUACCUUGAUAGUAGGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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