Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31611 | 3' | -56.8 | NC_006879.1 | + | 7916 | 0.66 | 0.453128 |
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Target: 5'- gACCGGUaagAGcGUGAGCC--AGGUGCACa -3' miRNA: 3'- -UGGUCG---UCcUACUCGGacUUCGCGUG- -5' |
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| 31611 | 3' | -56.8 | NC_006879.1 | + | 8902 | 0.66 | 0.453128 |
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Target: 5'- gACCGGCAGGcgGu-CCacGAAGCGCuCg -3' miRNA: 3'- -UGGUCGUCCuaCucGGa-CUUCGCGuG- -5' |
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| 31611 | 3' | -56.8 | NC_006879.1 | + | 17265 | 0.66 | 0.442867 |
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Target: 5'- aGCCGGCAGGggGGgaacggucgggcGCgUGggGgGCGg -3' miRNA: 3'- -UGGUCGUCCuaCU------------CGgACuuCgCGUg -5' |
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| 31611 | 3' | -56.8 | NC_006879.1 | + | 24050 | 0.66 | 0.412892 |
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Target: 5'- cCCAGCagcaagAGGGUGcuuccGGCUUGAAGCuCACu -3' miRNA: 3'- uGGUCG------UCCUAC-----UCGGACUUCGcGUG- -5' |
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| 31611 | 3' | -56.8 | NC_006879.1 | + | 6193 | 0.68 | 0.330922 |
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Target: 5'- cAUgGGguGGGUcaGGGCC-GAGGCGUACa -3' miRNA: 3'- -UGgUCguCCUA--CUCGGaCUUCGCGUG- -5' |
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| 31611 | 3' | -56.8 | NC_006879.1 | + | 4230 | 0.68 | 0.330082 |
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Target: 5'- uGCCcgacuGgGGGAugauaguuccaucUGGGCCUGGAGCGUAg -3' miRNA: 3'- -UGGu----CgUCCU-------------ACUCGGACUUCGCGUg -5' |
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| 31611 | 3' | -56.8 | NC_006879.1 | + | 22342 | 0.69 | 0.290832 |
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Target: 5'- uACUuGCGGGAgaUGAGCCccGAGCGCGa -3' miRNA: 3'- -UGGuCGUCCU--ACUCGGacUUCGCGUg -5' |
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| 31611 | 3' | -56.8 | NC_006879.1 | + | 11097 | 0.73 | 0.163186 |
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Target: 5'- cGCCGGCGGGAgcUGAGCgacagGGAGCugauGCACa -3' miRNA: 3'- -UGGUCGUCCU--ACUCGga---CUUCG----CGUG- -5' |
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| 31611 | 3' | -56.8 | NC_006879.1 | + | 4938 | 1.08 | 0.000331 |
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Target: 5'- cACCAGCAGGAUGAGCCUGAAGCGCACc -3' miRNA: 3'- -UGGUCGUCCUACUCGGACUUCGCGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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