Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31614 | 5' | -52.2 | NC_006879.1 | + | 16981 | 0.66 | 0.724448 |
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Target: 5'- ---cCGGugcgccUCCGAGAccGCagaAGCGACCCa -3' miRNA: 3'- auuaGUCu-----AGGUUCU--CGg--UCGCUGGG- -5' |
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| 31614 | 5' | -52.2 | NC_006879.1 | + | 33037 | 0.66 | 0.713049 |
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Target: 5'- cAAUCGcGGccUCCAaaAGAGCCuG-GACCCg -3' miRNA: 3'- aUUAGU-CU--AGGU--UCUCGGuCgCUGGG- -5' |
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| 31614 | 5' | -52.2 | NC_006879.1 | + | 4669 | 0.67 | 0.678361 |
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Target: 5'- cAGUCGG-UCCcAGAGCuCGGUGACg- -3' miRNA: 3'- aUUAGUCuAGGuUCUCG-GUCGCUGgg -5' |
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| 31614 | 5' | -52.2 | NC_006879.1 | + | 9746 | 0.67 | 0.643221 |
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Target: 5'- --cUCGGua-CAGGGGCCAGcCGACUg -3' miRNA: 3'- auuAGUCuagGUUCUCGGUC-GCUGGg -5' |
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| 31614 | 5' | -52.2 | NC_006879.1 | + | 11769 | 0.69 | 0.56136 |
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Target: 5'- --cUCAGAaaccgcUCCuucuGAGCCAGCG-CCUc -3' miRNA: 3'- auuAGUCU------AGGuu--CUCGGUCGCuGGG- -5' |
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| 31614 | 5' | -52.2 | NC_006879.1 | + | 20520 | 0.69 | 0.549844 |
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Target: 5'- uUAcgCGGGuUCCAGgcgaaggcGAGCCAGUgGACCCc -3' miRNA: 3'- -AUuaGUCU-AGGUU--------CUCGGUCG-CUGGG- -5' |
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| 31614 | 5' | -52.2 | NC_006879.1 | + | 23177 | 0.7 | 0.482587 |
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Target: 5'- gUGGUCgGGAUCCAGGGGCuCAGCu-CUCu -3' miRNA: 3'- -AUUAG-UCUAGGUUCUCG-GUCGcuGGG- -5' |
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| 31614 | 5' | -52.2 | NC_006879.1 | + | 8278 | 0.71 | 0.450466 |
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Target: 5'- --uUCAGGUUCAGGAcCCGGgGGCCg -3' miRNA: 3'- auuAGUCUAGGUUCUcGGUCgCUGGg -5' |
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| 31614 | 5' | -52.2 | NC_006879.1 | + | 1829 | 0.72 | 0.380408 |
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Target: 5'- ----gAGaAUCUGAGAGCCGGCcugGACCCu -3' miRNA: 3'- auuagUC-UAGGUUCUCGGUCG---CUGGG- -5' |
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| 31614 | 5' | -52.2 | NC_006879.1 | + | 10880 | 1.11 | 0.000753 |
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Target: 5'- uUAAUCAGAUCCAAGAGCCAGCGACCCu -3' miRNA: 3'- -AUUAGUCUAGGUUCUCGGUCGCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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