Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31615 | 3' | -53.3 | NC_006879.1 | + | 24235 | 0.66 | 0.687842 |
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Target: 5'- gGCcGUGAGGGGggCuUUC-GGUGGUCGa -3' miRNA: 3'- -CGuCGUUCCCCa-GcAAGuCCAUCAGC- -5' |
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| 31615 | 3' | -53.3 | NC_006879.1 | + | 17222 | 0.67 | 0.630517 |
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Target: 5'- gGCGGCGGGGGGagcucuagCGUgaCAGGcuugUAGUCc -3' miRNA: 3'- -CGUCGUUCCCCa-------GCAa-GUCC----AUCAGc -5' |
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| 31615 | 3' | -53.3 | NC_006879.1 | + | 19438 | 0.68 | 0.60063 |
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Target: 5'- gGCGGgGAuGGGGUacagcauguuggccgCGcaCAGGUAGUCGu -3' miRNA: 3'- -CGUCgUU-CCCCA---------------GCaaGUCCAUCAGC- -5' |
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| 31615 | 3' | -53.3 | NC_006879.1 | + | 6046 | 0.68 | 0.589178 |
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Target: 5'- -gGGCGGGGGGUCGgcgUCgaugguaaauaugccGGGUAG-Ca -3' miRNA: 3'- cgUCGUUCCCCAGCa--AG---------------UCCAUCaGc -5' |
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| 31615 | 3' | -53.3 | NC_006879.1 | + | 30441 | 0.7 | 0.474031 |
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Target: 5'- -uGGCAAGGGGUCGUUCAauGGc----- -3' miRNA: 3'- cgUCGUUCCCCAGCAAGU--CCaucagc -5' |
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| 31615 | 3' | -53.3 | NC_006879.1 | + | 12809 | 1.11 | 0.000679 |
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Target: 5'- cGCAGCAAGGGGUCGUUCAGGUAGUCGc -3' miRNA: 3'- -CGUCGUUCCCCAGCAAGUCCAUCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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