Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31615 | 5' | -58.5 | NC_006879.1 | + | 17625 | 0.65 | 0.471409 |
Target: 5'- gGGCGaacugcacgagACCG-GGGCucagguacuccgagGCGUccUGCCCGGCg -3' miRNA: 3'- -CCGU-----------UGGCgCCCG--------------CGCA--AUGGGCUGg -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 11844 | 0.66 | 0.464395 |
Target: 5'- uGC--CCGCgGGGCaCGUUGgCCGGCUg -3' miRNA: 3'- cCGuuGGCG-CCCGcGCAAUgGGCUGG- -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 17317 | 0.66 | 0.448571 |
Target: 5'- gGGCGGCUGgcagaggcacggcgaCGGGUGCGgacacggguCCCG-CCg -3' miRNA: 3'- -CCGUUGGC---------------GCCCGCGCaau------GGGCuGG- -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 21220 | 0.66 | 0.444661 |
Target: 5'- -aCAGCCGCGGGaGC----UCCGGCCc -3' miRNA: 3'- ccGUUGGCGCCCgCGcaauGGGCUGG- -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 33379 | 0.67 | 0.415968 |
Target: 5'- cGCGAUCGCGGaGaGCa--ACCUGACCu -3' miRNA: 3'- cCGUUGGCGCC-CgCGcaaUGGGCUGG- -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 23693 | 0.67 | 0.415968 |
Target: 5'- uGGCGACCGUGcGCuCGcugaaACCgGACCa -3' miRNA: 3'- -CCGUUGGCGCcCGcGCaa---UGGgCUGG- -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 16450 | 0.67 | 0.379529 |
Target: 5'- cGCcGCCGCcaucGGUGCcaUACCCGGCg -3' miRNA: 3'- cCGuUGGCGc---CCGCGcaAUGGGCUGg -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 19004 | 0.67 | 0.370759 |
Target: 5'- -cCAGCC-CGGGUgGCGUcACgCGACCg -3' miRNA: 3'- ccGUUGGcGCCCG-CGCAaUGgGCUGG- -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 16283 | 0.68 | 0.362129 |
Target: 5'- uGGUAACUGUcuuuccccGGCGUGUUACgCGGCg -3' miRNA: 3'- -CCGUUGGCGc-------CCGCGCAAUGgGCUGg -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 16218 | 0.68 | 0.353639 |
Target: 5'- cGGCGGCgGCGacggguucggcGGCGgGUU-CCUGACg -3' miRNA: 3'- -CCGUUGgCGC-----------CCGCgCAAuGGGCUGg -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 9505 | 0.68 | 0.353639 |
Target: 5'- uGCAGCCcuGCGGGgGUucgGCUCGACUc -3' miRNA: 3'- cCGUUGG--CGCCCgCGcaaUGGGCUGG- -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 15117 | 0.68 | 0.337085 |
Target: 5'- aGCAGCCugGCGGGCagccugucgGCGggcccuCCCGGCg -3' miRNA: 3'- cCGUUGG--CGCCCG---------CGCaau---GGGCUGg -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 7821 | 0.69 | 0.313327 |
Target: 5'- cGGCAcCCG-GGGCGuCGUcguCCCcACCg -3' miRNA: 3'- -CCGUuGGCgCCCGC-GCAau-GGGcUGG- -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 25288 | 0.7 | 0.256251 |
Target: 5'- gGGCcuGGCCGCGGGgGCc--GCCCagGACUa -3' miRNA: 3'- -CCG--UUGGCGCCCgCGcaaUGGG--CUGG- -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 22870 | 0.71 | 0.207978 |
Target: 5'- -uCGGCCGCGGuGCG-GUUgGCCCGGCa -3' miRNA: 3'- ccGUUGGCGCC-CGCgCAA-UGGGCUGg -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 6334 | 0.72 | 0.197204 |
Target: 5'- aGCAuCCGCgggGGGCGCuGUUACCCcACUu -3' miRNA: 3'- cCGUuGGCG---CCCGCG-CAAUGGGcUGG- -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 15554 | 0.72 | 0.181468 |
Target: 5'- cGGcCGACCGCucucccgGGGUaaaaacuaccGCGgUGCCCGGCCu -3' miRNA: 3'- -CC-GUUGGCG-------CCCG----------CGCaAUGGGCUGG- -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 5964 | 0.77 | 0.076531 |
Target: 5'- -aCAACCGCGgcuGGCGCGUgcgcauccUGCCCGACg -3' miRNA: 3'- ccGUUGGCGC---CCGCGCA--------AUGGGCUGg -5' |
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31615 | 5' | -58.5 | NC_006879.1 | + | 12774 | 1.14 | 0.00013 |
Target: 5'- cGGCAACCGCGGGCGCGUUACCCGACCa -3' miRNA: 3'- -CCGUUGGCGCCCGCGCAAUGGGCUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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