Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31616 | 5' | -55.9 | NC_006879.1 | + | 8269 | 0.66 | 0.505747 |
Target: 5'- aGGACccgGGGGCCgGUGGUCUucacgcagagacGGCACaUCCa -3' miRNA: 3'- -UUUGa--CCCUGG-CGCUAGG------------UCGUG-AGG- -5' |
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31616 | 5' | -55.9 | NC_006879.1 | + | 17841 | 0.66 | 0.494986 |
Target: 5'- -uACUcGGGuACCGCGuacaauUCCcuggcucccaagGGCGCUCCc -3' miRNA: 3'- uuUGA-CCC-UGGCGCu-----AGG------------UCGUGAGG- -5' |
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31616 | 5' | -55.9 | NC_006879.1 | + | 14667 | 0.67 | 0.439842 |
Target: 5'- gGGACUGGGcuccggcaagauguACgGCGGagCCaaaaGGCGCUCCa -3' miRNA: 3'- -UUUGACCC--------------UGgCGCUa-GG----UCGUGAGG- -5' |
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31616 | 5' | -55.9 | NC_006879.1 | + | 24662 | 0.68 | 0.413126 |
Target: 5'- gGGGCgucgGGGGCCGgGGcUUCGGCugUCg -3' miRNA: 3'- -UUUGa---CCCUGGCgCU-AGGUCGugAGg -5' |
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31616 | 5' | -55.9 | NC_006879.1 | + | 11340 | 0.68 | 0.384646 |
Target: 5'- cGGACgGGGccACCGCgGAUCCcGCACUUUu -3' miRNA: 3'- -UUUGaCCC--UGGCG-CUAGGuCGUGAGG- -5' |
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31616 | 5' | -55.9 | NC_006879.1 | + | 15126 | 0.69 | 0.366395 |
Target: 5'- -uACUGGGugCGCGAUUCugugacGGgAgUCCg -3' miRNA: 3'- uuUGACCCugGCGCUAGG------UCgUgAGG- -5' |
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31616 | 5' | -55.9 | NC_006879.1 | + | 20819 | 0.7 | 0.315308 |
Target: 5'- cGACcugGGGGCUGUGAagcaugcugUUgGGCACUCCg -3' miRNA: 3'- uUUGa--CCCUGGCGCU---------AGgUCGUGAGG- -5' |
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31616 | 5' | -55.9 | NC_006879.1 | + | 21723 | 0.71 | 0.284345 |
Target: 5'- -uGCUGGGuACUGCGAugcUCCcauacGGCGCaUCCg -3' miRNA: 3'- uuUGACCC-UGGCGCU---AGG-----UCGUG-AGG- -5' |
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31616 | 5' | -55.9 | NC_006879.1 | + | 17867 | 1.08 | 0.000464 |
Target: 5'- aAAACUGGGACCGCGAUCCAGCACUCCc -3' miRNA: 3'- -UUUGACCCUGGCGCUAGGUCGUGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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