Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31617 | 3' | -56.8 | NC_006879.1 | + | 7850 | 0.66 | 0.490028 |
Target: 5'- uGUCCAGGGAGUcgAGGGCggCCGC-GCUgAg -3' miRNA: 3'- -CGGGUUUUUCA--UCCCG--GGCGuCGGgU- -5' |
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31617 | 3' | -56.8 | NC_006879.1 | + | 21175 | 0.66 | 0.479445 |
Target: 5'- cGCCCGAcgucaUGGGaGCCCuGCAGCUg- -3' miRNA: 3'- -CGGGUUuuuc-AUCC-CGGG-CGUCGGgu -5' |
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31617 | 3' | -56.8 | NC_006879.1 | + | 5912 | 0.66 | 0.458625 |
Target: 5'- cCCCG---AGUGGcGGUgCGUGGCCCGc -3' miRNA: 3'- cGGGUuuuUCAUC-CCGgGCGUCGGGU- -5' |
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31617 | 3' | -56.8 | NC_006879.1 | + | 10517 | 0.66 | 0.448397 |
Target: 5'- aGCCCGAcGAGcguucUGGGGUgaGCccGGCCCGc -3' miRNA: 3'- -CGGGUUuUUC-----AUCCCGggCG--UCGGGU- -5' |
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31617 | 3' | -56.8 | NC_006879.1 | + | 19982 | 0.66 | 0.446367 |
Target: 5'- uGCCCcucucgcauGGUGGGuCCCauGUAGCCCAc -3' miRNA: 3'- -CGGGuuuu-----UCAUCCcGGG--CGUCGGGU- -5' |
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31617 | 3' | -56.8 | NC_006879.1 | + | 13408 | 0.66 | 0.438297 |
Target: 5'- cCCCGAcGGAGgcGGGCaCGCAGaCCAu -3' miRNA: 3'- cGGGUU-UUUCauCCCGgGCGUCgGGU- -5' |
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31617 | 3' | -56.8 | NC_006879.1 | + | 22462 | 0.67 | 0.428329 |
Target: 5'- cGCCCGAGGaucuGGaGGGGCCUGacCGGCgCGa -3' miRNA: 3'- -CGGGUUUU----UCaUCCCGGGC--GUCGgGU- -5' |
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31617 | 3' | -56.8 | NC_006879.1 | + | 9537 | 0.67 | 0.398299 |
Target: 5'- gGCCgAGAGGGUcGuGGCCgaccuagcguugcUGCAGCCCu -3' miRNA: 3'- -CGGgUUUUUCAuC-CCGG-------------GCGUCGGGu -5' |
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31617 | 3' | -56.8 | NC_006879.1 | + | 20627 | 0.68 | 0.371458 |
Target: 5'- aCCCAAAcGGUccaGGGGCCCcucuCGGCCg- -3' miRNA: 3'- cGGGUUUuUCA---UCCCGGGc---GUCGGgu -5' |
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31617 | 3' | -56.8 | NC_006879.1 | + | 25446 | 0.69 | 0.304022 |
Target: 5'- aCCCGccuAGUu-GGCCCGCcGCCCAg -3' miRNA: 3'- cGGGUuuuUCAucCCGGGCGuCGGGU- -5' |
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31617 | 3' | -56.8 | NC_006879.1 | + | 14993 | 0.71 | 0.219338 |
Target: 5'- gGCCCGGAGGGUAGGcagaagggcuaugcGCCgCGCuGCCg- -3' miRNA: 3'- -CGGGUUUUUCAUCC--------------CGG-GCGuCGGgu -5' |
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31617 | 3' | -56.8 | NC_006879.1 | + | 19573 | 0.73 | 0.158306 |
Target: 5'- aGCCgGAguagacGAAGUAGGGgucgaaCCCGgAGCCCAg -3' miRNA: 3'- -CGGgUU------UUUCAUCCC------GGGCgUCGGGU- -5' |
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31617 | 3' | -56.8 | NC_006879.1 | + | 20400 | 1.1 | 0.000258 |
Target: 5'- uGCCCAAAAAGUAGGGCCCGCAGCCCAg -3' miRNA: 3'- -CGGGUUUUUCAUCCCGGGCGUCGGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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