miRNA: 3'- -CCGUCg-------AGGUAAUGGGGgaUGAaCGc -5'
miRNA: 3'- -CCGUCG------AGGuaauGGGGGAUGa-ACGC- -5'
miRNA: 3'- -CCGUCGa-GGuaAUGGGGGa----------UGAACGC- -5'
miRNA: 3'- -CCGUCGAgGUAauGGGGGAUGa-----ACGC- -5'
miRNA: 3'- -CCGUCGAGGUAAUGGGGGAUGAACGC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31618 | 3' | -54 | NC_006879.1 | + | 13253 | 0.68 | 0.531543 |
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Target: 5'- gGGCAGaaacagcaUCCGUUACUCCgaGCUgGCa -3' miRNA: 3'- -CCGUCg-------AGGUAAUGGGGgaUGAaCGc -5' |
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| 31618 | 3' | -54 | NC_006879.1 | + | 21434 | 0.68 | 0.498518 |
|
Target: 5'- gGGCAGCgacgcaUCCugauCCCUCUGCgcUGCGa -3' miRNA: 3'- -CCGUCG------AGGuaauGGGGGAUGa-ACGC- -5' |
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| 31618 | 3' | -54 | NC_006879.1 | + | 2648 | 0.69 | 0.46644 |
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Target: 5'- cGGCAGCggCCugUGCCCCUcgcagcucuuaACUUGCGc -3' miRNA: 3'- -CCGUCGa-GGuaAUGGGGGa----------UGAACGC- -5' |
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| 31618 | 3' | -54 | NC_006879.1 | + | 7880 | 0.7 | 0.425405 |
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Target: 5'- gGGCAGCUgCGUccCCUCCUGCaacgccUGCGc -3' miRNA: 3'- -CCGUCGAgGUAauGGGGGAUGa-----ACGC- -5' |
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| 31618 | 3' | -54 | NC_006879.1 | + | 22361 | 1.12 | 0.000394 |
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Target: 5'- aGGCAGCUCCAUUACCCCCUACUUGCGg -3' miRNA: 3'- -CCGUCGAGGUAAUGGGGGAUGAACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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