Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31618 | 5' | -62.1 | NC_006879.1 | + | 8427 | 0.66 | 0.253807 |
Target: 5'- uGGaGCGAGCGGGccacGGCGaCCUCcGCg- -3' miRNA: 3'- -UCgCGCUCGUCCu---CCGC-GGAGuCGag -5' |
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31618 | 5' | -62.1 | NC_006879.1 | + | 20164 | 0.66 | 0.247302 |
Target: 5'- aGGCGUGAGCGGaGuuGGCGUa-CAGCa- -3' miRNA: 3'- -UCGCGCUCGUC-Cu-CCGCGgaGUCGag -5' |
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31618 | 5' | -62.1 | NC_006879.1 | + | 24622 | 0.66 | 0.234707 |
Target: 5'- aGGCGCGcuggauGCAGGuGGUuuuucuucggucGCCUCuuccucugaaAGCUCg -3' miRNA: 3'- -UCGCGCu-----CGUCCuCCG------------CGGAG----------UCGAG- -5' |
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31618 | 5' | -62.1 | NC_006879.1 | + | 11184 | 0.66 | 0.228614 |
Target: 5'- cAGCGCccucuGCAGGcuGUGCaUCAGCUCc -3' miRNA: 3'- -UCGCGcu---CGUCCucCGCGgAGUCGAG- -5' |
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31618 | 5' | -62.1 | NC_006879.1 | + | 21536 | 0.67 | 0.205567 |
Target: 5'- cGCGC-AGCAGcGGGCGUCUUcGUUCu -3' miRNA: 3'- uCGCGcUCGUCcUCCGCGGAGuCGAG- -5' |
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31618 | 5' | -62.1 | NC_006879.1 | + | 22225 | 0.67 | 0.184559 |
Target: 5'- gAGgGCGAaggcaccguGguGGAGGUggGCUUCAGCUa -3' miRNA: 3'- -UCgCGCU---------CguCCUCCG--CGGAGUCGAg -5' |
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31618 | 5' | -62.1 | NC_006879.1 | + | 20835 | 0.68 | 0.160982 |
Target: 5'- cGGCGCaGGguGGGGGCGaCCUgggGGCUg -3' miRNA: 3'- -UCGCGcUCguCCUCCGC-GGAg--UCGAg -5' |
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31618 | 5' | -62.1 | NC_006879.1 | + | 13196 | 0.68 | 0.156604 |
Target: 5'- cGUGCcGGCcacgcuGGAGGCGCCUUAcGUUCc -3' miRNA: 3'- uCGCGcUCGu-----CCUCCGCGGAGU-CGAG- -5' |
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31618 | 5' | -62.1 | NC_006879.1 | + | 120 | 0.69 | 0.152335 |
Target: 5'- gGGCGgGAGUgGGGAGGCG-CUUAGUUUu -3' miRNA: 3'- -UCGCgCUCG-UCCUCCGCgGAGUCGAG- -5' |
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31618 | 5' | -62.1 | NC_006879.1 | + | 8836 | 0.69 | 0.152335 |
Target: 5'- gGGCGCuuucGAGCAGGAGGUGCgCgacaCGGUc- -3' miRNA: 3'- -UCGCG----CUCGUCCUCCGCG-Ga---GUCGag -5' |
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31618 | 5' | -62.1 | NC_006879.1 | + | 22255 | 0.69 | 0.144112 |
Target: 5'- gGGaGgGAGCu-GAGGCGCCUCcuGCUCg -3' miRNA: 3'- -UCgCgCUCGucCUCCGCGGAGu-CGAG- -5' |
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31618 | 5' | -62.1 | NC_006879.1 | + | 4865 | 0.69 | 0.140154 |
Target: 5'- gGGCGCuuGCgAGGGuGCGCUUCAGgCUCa -3' miRNA: 3'- -UCGCGcuCG-UCCUcCGCGGAGUC-GAG- -5' |
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31618 | 5' | -62.1 | NC_006879.1 | + | 11803 | 0.71 | 0.105739 |
Target: 5'- -cCGCGGGCAGGAGGacuacgugaGCUUCAucagcGCUCu -3' miRNA: 3'- ucGCGCUCGUCCUCCg--------CGGAGU-----CGAG- -5' |
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31618 | 5' | -62.1 | NC_006879.1 | + | 10862 | 0.73 | 0.074926 |
Target: 5'- -uCGUGGuGCAGGAgaGGgGCCUCAGCUUa -3' miRNA: 3'- ucGCGCU-CGUCCU--CCgCGGAGUCGAG- -5' |
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31618 | 5' | -62.1 | NC_006879.1 | + | 22321 | 1.08 | 0.00011 |
Target: 5'- gAGCGCGAGCAGGAGGCGCCUCAGCUCc -3' miRNA: 3'- -UCGCGCUCGUCCUCCGCGGAGUCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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