Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31619 | 3' | -53.6 | NC_006879.1 | + | 9808 | 0.66 | 0.66101 |
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Target: 5'- -cGAUGGUAGUG--GUAGAUGUaGCGg -3' miRNA: 3'- gaCUGCCGUCACuaCGUCUACAgCGU- -5' |
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| 31619 | 3' | -53.6 | NC_006879.1 | + | 24984 | 0.66 | 0.637787 |
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Target: 5'- -cGGCGGCAGcgGcgGCGGAguuuuuccUCGCGg -3' miRNA: 3'- gaCUGCCGUCa-CuaCGUCUac------AGCGU- -5' |
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| 31619 | 3' | -53.6 | NC_006879.1 | + | 14999 | 0.66 | 0.626161 |
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Target: 5'- aUGGCGGCGGUGggGC-GAcGUCuccgauGCAg -3' miRNA: 3'- gACUGCCGUCACuaCGuCUaCAG------CGU- -5' |
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| 31619 | 3' | -53.6 | NC_006879.1 | + | 20270 | 0.69 | 0.479218 |
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Target: 5'- aUGACGccGCGGUGcgGCuGGUGgaugCGCAc -3' miRNA: 3'- gACUGC--CGUCACuaCGuCUACa---GCGU- -5' |
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| 31619 | 3' | -53.6 | NC_006879.1 | + | 12955 | 0.69 | 0.468539 |
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Target: 5'- uCUGACGacgcCAGUGcgGCGGAaccagcgGUCGCGg -3' miRNA: 3'- -GACUGCc---GUCACuaCGUCUa------CAGCGU- -5' |
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| 31619 | 3' | -53.6 | NC_006879.1 | + | 10275 | 0.75 | 0.205404 |
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Target: 5'- aCUGccgccGCGGCGGUGGUGguGGgcgucugagGUCGCAu -3' miRNA: 3'- -GAC-----UGCCGUCACUACguCUa--------CAGCGU- -5' |
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| 31619 | 3' | -53.6 | NC_006879.1 | + | 22213 | 0.75 | 0.194134 |
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Target: 5'- -gGAUGGCGGcGgcGCGGGUGUCGCc -3' miRNA: 3'- gaCUGCCGUCaCuaCGUCUACAGCGu -5' |
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| 31619 | 3' | -53.6 | NC_006879.1 | + | 26029 | 1.09 | 0.000707 |
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Target: 5'- gCUGACGGCAGUGAUGCAGAUGUCGCAg -3' miRNA: 3'- -GACUGCCGUCACUACGUCUACAGCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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