Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31620 | 3' | -52.3 | NC_006879.1 | + | 10618 | 0.66 | 0.703804 |
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Target: 5'- aCGCGCUcaccagguUGGCCGcCGagacgUGAGCGCGGg -3' miRNA: 3'- -GCGUGG--------ACCGGUuGUaa---ACUCGUGUU- -5' |
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| 31620 | 3' | -52.3 | NC_006879.1 | + | 10548 | 0.66 | 0.668515 |
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Target: 5'- cCGCGCUcacgucucggcGGCCAACcuggUGAGCGCGu -3' miRNA: 3'- -GCGUGGa----------CCGGUUGuaa-ACUCGUGUu -5' |
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| 31620 | 3' | -52.3 | NC_006879.1 | + | 23660 | 0.67 | 0.632866 |
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Target: 5'- aGCGCuCUGGCgGACAUcaucuucccgGAGCGCc- -3' miRNA: 3'- gCGUG-GACCGgUUGUAaa--------CUCGUGuu -5' |
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| 31620 | 3' | -52.3 | NC_006879.1 | + | 11783 | 0.67 | 0.632866 |
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Target: 5'- aCGCAgCcGGCCAACGUgccccgcGGGCAgGAg -3' miRNA: 3'- -GCGUgGaCCGGUUGUAaa-----CUCGUgUU- -5' |
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| 31620 | 3' | -52.3 | NC_006879.1 | + | 2831 | 0.68 | 0.550193 |
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Target: 5'- aGCACCUaggugaacGGCgCAACGcaUGAGCAUAu -3' miRNA: 3'- gCGUGGA--------CCG-GUUGUaaACUCGUGUu -5' |
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| 31620 | 3' | -52.3 | NC_006879.1 | + | 2738 | 1.07 | 0.001196 |
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Target: 5'- cCGCACCUGGCCAACAUUUGAGCACAAu -3' miRNA: 3'- -GCGUGGACCGGUUGUAAACUCGUGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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