Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31622 | 3' | -45 | NC_006879.1 | + | 94 | 0.66 | 0.993499 |
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Target: 5'- gGGUGacgcGGGGUggCGcgAGGGCGgGGCg -3' miRNA: 3'- gCUACa---UUUCAa-GCuaUUUCGCgCCG- -5' |
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| 31622 | 3' | -45 | NC_006879.1 | + | 21831 | 0.68 | 0.972784 |
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Target: 5'- uCGGUG-AAGGUcuucuggUUGGUGAAGguuaGCGGCa -3' miRNA: 3'- -GCUACaUUUCA-------AGCUAUUUCg---CGCCG- -5' |
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| 31622 | 3' | -45 | NC_006879.1 | + | 3072 | 0.71 | 0.904711 |
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Target: 5'- gCGAUGaGGAGUgaGGUGAGGgGCGGa -3' miRNA: 3'- -GCUACaUUUCAagCUAUUUCgCGCCg -5' |
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| 31622 | 3' | -45 | NC_006879.1 | + | 25854 | 0.71 | 0.889214 |
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Target: 5'- aCGAacUGgaGAGUgcCGAUucccccGGAGCGCGGCu -3' miRNA: 3'- -GCU--ACauUUCAa-GCUA------UUUCGCGCCG- -5' |
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| 31622 | 3' | -45 | NC_006879.1 | + | 26166 | 0.72 | 0.880982 |
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Target: 5'- gGcgGUGAAGgugUCGAUGAGGCGagacuCGaGCg -3' miRNA: 3'- gCuaCAUUUCa--AGCUAUUUCGC-----GC-CG- -5' |
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| 31622 | 3' | -45 | NC_006879.1 | + | 22707 | 0.72 | 0.869812 |
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Target: 5'- gCGAgaaAAGGUUCGccucguaggccugggAGAGCGCGGCg -3' miRNA: 3'- -GCUacaUUUCAAGCua-------------UUUCGCGCCG- -5' |
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| 31622 | 3' | -45 | NC_006879.1 | + | 20295 | 0.73 | 0.825333 |
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Target: 5'- aGGUGUGcgcAGGUagacggccUCGAUGAcgccgcGGUGCGGCu -3' miRNA: 3'- gCUACAU---UUCA--------AGCUAUU------UCGCGCCG- -5' |
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| 31622 | 3' | -45 | NC_006879.1 | + | 10016 | 0.74 | 0.772036 |
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Target: 5'- gGggGUAAAG-UCGcaAGGGUGCGGCg -3' miRNA: 3'- gCuaCAUUUCaAGCuaUUUCGCGCCG- -5' |
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| 31622 | 3' | -45 | NC_006879.1 | + | 7045 | 1.15 | 0.003221 |
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Target: 5'- aCGAUGUAAAGUUCGAUAAAGCGCGGCu -3' miRNA: 3'- -GCUACAUUUCAAGCUAUUUCGCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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