Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31624 | 3' | -57.8 | NC_006879.1 | + | 23261 | 0.66 | 0.502628 |
Target: 5'- gCCGUGCuGGUCaccgugGAGCUGgAAUgCCUGCa -3' miRNA: 3'- -GGUACGcUCAG------CUCGGC-UUGgGGGCG- -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 24862 | 0.66 | 0.501587 |
Target: 5'- aCCGUGaUGGaagaggagguagcGUCGGGCaaaggaGAuguuuCCCCCGCa -3' miRNA: 3'- -GGUAC-GCU-------------CAGCUCGg-----CUu----GGGGGCG- -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 3255 | 0.66 | 0.492258 |
Target: 5'- aCCGUGgacGGaCG-GCCGGucGCCCCUGCa -3' miRNA: 3'- -GGUACgc-UCaGCuCGGCU--UGGGGGCG- -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 10051 | 0.66 | 0.451824 |
Target: 5'- cCCGguuCGAGaCcGGCCGGAUCCgCCGCu -3' miRNA: 3'- -GGUac-GCUCaGcUCGGCUUGGG-GGCG- -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 9819 | 0.66 | 0.451824 |
Target: 5'- cCCcgGCGcccccgccacAGUCG-GCUG-GCCCCUGUa -3' miRNA: 3'- -GGuaCGC----------UCAGCuCGGCuUGGGGGCG- -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 21157 | 0.67 | 0.422722 |
Target: 5'- ---cGC-AGUUaGGGCCgGAGCUCCCGCg -3' miRNA: 3'- gguaCGcUCAG-CUCGG-CUUGGGGGCG- -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 6116 | 0.67 | 0.40211 |
Target: 5'- aCCcgGCauauuuaccaUCGAcGCCGAcCCCCCGCc -3' miRNA: 3'- -GGuaCGcuc-------AGCU-CGGCUuGGGGGCG- -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 26017 | 0.67 | 0.394775 |
Target: 5'- -gAUGCaGAuGUCGcAGCCGAGCUCucacggCCGCa -3' miRNA: 3'- ggUACG-CU-CAGC-UCGGCUUGGG------GGCG- -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 4789 | 0.68 | 0.359423 |
Target: 5'- aCA-GCGAaaGUCG-GCCcAACCCCCGa -3' miRNA: 3'- gGUaCGCU--CAGCuCGGcUUGGGGGCg -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 10569 | 0.68 | 0.359423 |
Target: 5'- cCCAgaacGCucGUCGGGCuCGAAauCCUCCGCc -3' miRNA: 3'- -GGUa---CGcuCAGCUCG-GCUU--GGGGGCG- -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 4036 | 0.68 | 0.350938 |
Target: 5'- aCCGUGCuGGU--GGUCcuucauaacauGAACCCCCGCa -3' miRNA: 3'- -GGUACGcUCAgcUCGG-----------CUUGGGGGCG- -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 9545 | 0.68 | 0.34842 |
Target: 5'- gCCGUGCuggccgagaGGGUCGuGGCCGAccuagcguugcugcAgCCCUGCg -3' miRNA: 3'- -GGUACG---------CUCAGC-UCGGCU--------------UgGGGGCG- -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 12932 | 0.69 | 0.326344 |
Target: 5'- aCCA-GCG-GUCGcGGCUGAcuguccucccACUCCCGCu -3' miRNA: 3'- -GGUaCGCuCAGC-UCGGCU----------UGGGGGCG- -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 19933 | 0.69 | 0.310671 |
Target: 5'- aCCAUGCGAGagGGGCa-GGCCuaCCCGg -3' miRNA: 3'- -GGUACGCUCagCUCGgcUUGG--GGGCg -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 20510 | 0.7 | 0.274016 |
Target: 5'- uCCAgGCGAaggCGAGCCaguGGACCCCCcCg -3' miRNA: 3'- -GGUaCGCUca-GCUCGG---CUUGGGGGcG- -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 8431 | 0.74 | 0.140407 |
Target: 5'- aCCGUggaGCGAG-CGGGCCacggcGACCUCCGCg -3' miRNA: 3'- -GGUA---CGCUCaGCUCGGc----UUGGGGGCG- -5' |
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31624 | 3' | -57.8 | NC_006879.1 | + | 9436 | 1.14 | 0.000172 |
Target: 5'- gCCAUGCGAGUCGAGCCGAACCCCCGCa -3' miRNA: 3'- -GGUACGCUCAGCUCGGCUUGGGGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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