miRNA display CGI


Results 1 - 5 of 5 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
31625 3' -53.9 NC_006879.1 + 23243 0.66 0.59732
Target:  5'- cGCAAGaugaugaUGGCGGCCguGCUGgUCa -3'
miRNA:   3'- -CGUUUggag---ACUGCCGGguCGACaAG- -5'
31625 3' -53.9 NC_006879.1 + 11648 0.66 0.632073
Target:  5'- aGCccGCCUCgucgcggcgGAUGGCCUuGCUGUc- -3'
miRNA:   3'- -CGuuUGGAGa--------CUGCCGGGuCGACAag -5'
31625 3' -53.9 NC_006879.1 + 15030 0.67 0.585787
Target:  5'- gGCccGCCUCaGcACGGCCCuGGCUGc-- -3'
miRNA:   3'- -CGuuUGGAGaC-UGCCGGG-UCGACaag -5'
31625 3' -53.9 NC_006879.1 + 11961 0.67 0.585787
Target:  5'- uGCAAGCCcugucgggacgUCUGGaagaaguaaucCGGCCCAGaCUGa-- -3'
miRNA:   3'- -CGUUUGG-----------AGACU-----------GCCGGGUC-GACaag -5'
31625 3' -53.9 NC_006879.1 + 10726 1.11 0.00047
Target:  5'- aGCAAACCUCUGACGGCCCAGCUGUUCc -3'
miRNA:   3'- -CGUUUGGAGACUGCCGGGUCGACAAG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.