Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31630 | 3' | -59.2 | NC_006879.1 | + | 25012 | 0.66 | 0.342093 |
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Target: 5'- --aGCUCuucggugGCCGguGGAuccuuGGCGGCgGCAg -3' miRNA: 3'- auaCGAG-------CGGCguUCU-----CCGCCGaCGU- -5' |
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| 31630 | 3' | -59.2 | NC_006879.1 | + | 10274 | 0.66 | 0.334497 |
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Target: 5'- -cUGC-CGCCGCGgcGGuGGUGGUggGCGu -3' miRNA: 3'- auACGaGCGGCGU--UCuCCGCCGa-CGU- -5' |
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| 31630 | 3' | -59.2 | NC_006879.1 | + | 11217 | 0.68 | 0.251672 |
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Target: 5'- --aGCgCGCgCGCcuuGGAGGCGGCggGCu -3' miRNA: 3'- auaCGaGCG-GCGu--UCUCCGCCGa-CGu -5' |
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| 31630 | 3' | -59.2 | NC_006879.1 | + | 8055 | 0.69 | 0.214058 |
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Target: 5'- --gGCgagCGCCGgGggguAGAGGCGGCucUGCGc -3' miRNA: 3'- auaCGa--GCGGCgU----UCUCCGCCG--ACGU- -5' |
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| 31630 | 3' | -59.2 | NC_006879.1 | + | 10010 | 0.69 | 0.202636 |
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Target: 5'- --aGCUCgggggguaaaGUCGCAAGGGuGCGGCgGCGg -3' miRNA: 3'- auaCGAG----------CGGCGUUCUC-CGCCGaCGU- -5' |
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| 31630 | 3' | -59.2 | NC_006879.1 | + | 22742 | 0.72 | 0.11164 |
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Target: 5'- cGUGUUCGCCGUAccAGAGGC-GCUGg- -3' miRNA: 3'- aUACGAGCGGCGU--UCUCCGcCGACgu -5' |
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| 31630 | 3' | -59.2 | NC_006879.1 | + | 3367 | 0.73 | 0.105318 |
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Target: 5'- -cUGCUCGCgGCAu--GGCGGCUGa- -3' miRNA: 3'- auACGAGCGgCGUucuCCGCCGACgu -5' |
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| 31630 | 3' | -59.2 | NC_006879.1 | + | 25946 | 0.73 | 0.099333 |
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Target: 5'- aUGUGCg-GCCGUGAGAGcuCGGCUGCGa -3' miRNA: 3'- -AUACGagCGGCGUUCUCc-GCCGACGU- -5' |
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| 31630 | 3' | -59.2 | NC_006879.1 | + | 14913 | 1.06 | 0.000253 |
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Target: 5'- cUAUGCUCGCCGCAAGAGGCGGCUGCAu -3' miRNA: 3'- -AUACGAGCGGCGUUCUCCGCCGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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