Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31630 | 5' | -63.4 | NC_006879.1 | + | 20643 | 0.69 | 0.140016 |
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Target: 5'- gGCCCCucuCgGCCGCC-UGCgGGCucuucuguuGCa -3' miRNA: 3'- gCGGGGu--GgCGGCGGuACGaCCGu--------CG- -5' |
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| 31630 | 5' | -63.4 | NC_006879.1 | + | 6283 | 0.7 | 0.115452 |
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Target: 5'- gCGCCCC-CCGCgG--AUGCUGGCucGCa -3' miRNA: 3'- -GCGGGGuGGCGgCggUACGACCGu-CG- -5' |
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| 31630 | 5' | -63.4 | NC_006879.1 | + | 23529 | 0.7 | 0.100433 |
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Target: 5'- -cCCUCACCuggcagaCCGCCAUGggcguCUGGCAGCa -3' miRNA: 3'- gcGGGGUGGc------GGCGGUAC-----GACCGUCG- -5' |
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| 31630 | 5' | -63.4 | NC_006879.1 | + | 15031 | 0.7 | 0.099593 |
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Target: 5'- gGgCCCGCCucagcacggcccugGCUGCCA-GCaUGGCGGCg -3' miRNA: 3'- gCgGGGUGG--------------CGGCGGUaCG-ACCGUCG- -5' |
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| 31630 | 5' | -63.4 | NC_006879.1 | + | 23531 | 0.71 | 0.097385 |
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Target: 5'- aGUCCCGCCGCgccuCGCCuugcaagGUGCaGuGCAGCa -3' miRNA: 3'- gCGGGGUGGCG----GCGG-------UACGaC-CGUCG- -5' |
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| 31630 | 5' | -63.4 | NC_006879.1 | + | 22105 | 0.72 | 0.077719 |
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Target: 5'- gGCCCCuCUaCCGCCGUGUUGGUgaagaaccccgacAGCa -3' miRNA: 3'- gCGGGGuGGcGGCGGUACGACCG-------------UCG- -5' |
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| 31630 | 5' | -63.4 | NC_006879.1 | + | 10213 | 0.73 | 0.069568 |
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Target: 5'- aCGCCcaccaCCACCGCCGCg--GC-GGCAGUa -3' miRNA: 3'- -GCGG-----GGUGGCGGCGguaCGaCCGUCG- -5' |
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| 31630 | 5' | -63.4 | NC_006879.1 | + | 11210 | 0.73 | 0.058613 |
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Target: 5'- gCGUCCCAgCGCgcgCGCCuUGgaGGCGGCg -3' miRNA: 3'- -GCGGGGUgGCG---GCGGuACgaCCGUCG- -5' |
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| 31630 | 5' | -63.4 | NC_006879.1 | + | 14949 | 0.98 | 0.000604 |
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Target: 5'- uCGCCCCACCGCCGCCAUGCU-GCAGCc -3' miRNA: 3'- -GCGGGGUGGCGGCGGUACGAcCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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