miRNA: 3'- gCGGaGG-CU-------CUGGCGUCuuCGcUGGG- -5'
miRNA: 3'- -GCGGaGGcuCUgGC-GUCUUCGCUGGG- -5'
miRNA: 3'- -GCGGAGGCUcUGGCgUCuuCGCUGgg -5'
miRNA: 3'- -GCGGAGGCU-CUGGCgUCuUCGCUGGg -5'
miRNA: 3'- -GCGGAGGCUCUGGCGUCUUCGCUGGG- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31631 | 3' | -58.7 | NC_006879.1 | + | 25278 | 0.71 | 0.200793 |
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Target: 5'- aGCC-CCaGAugggccuGGCCGCGGggGCcGCCCa -3' miRNA: 3'- gCGGaGG-CU-------CUGGCGUCuuCGcUGGG- -5' |
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| 31631 | 3' | -58.7 | NC_006879.1 | + | 16028 | 0.71 | 0.196011 |
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Target: 5'- cCGCC-CCucGG-CGuCAGAGGCGACCCg -3' miRNA: 3'- -GCGGaGGcuCUgGC-GUCUUCGCUGGG- -5' |
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| 31631 | 3' | -58.7 | NC_006879.1 | + | 16084 | 0.72 | 0.162121 |
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Target: 5'- uCGCCUCUGAcGCCG-AGggGCGGCg- -3' miRNA: 3'- -GCGGAGGCUcUGGCgUCuuCGCUGgg -5' |
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| 31631 | 3' | -58.7 | NC_006879.1 | + | 11403 | 0.74 | 0.116133 |
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Target: 5'- cCGCCUCCGAuGACUG-GGcGGCGGCCa -3' miRNA: 3'- -GCGGAGGCU-CUGGCgUCuUCGCUGGg -5' |
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| 31631 | 3' | -58.7 | NC_006879.1 | + | 16986 | 1.12 | 0.000169 |
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Target: 5'- gCGCCUCCGAGACCGCAGAAGCGACCCa -3' miRNA: 3'- -GCGGAGGCUCUGGCGUCUUCGCUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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