Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31637 | 5' | -54.8 | NC_006879.1 | + | 20337 | 1.14 | 0.000283 |
Target: 5'- aCCUGGGCUGCGGGCCCUACUUUUUGGg -3' miRNA: 3'- -GGACCCGACGCCCGGGAUGAAAAACC- -5' |
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31637 | 5' | -54.8 | NC_006879.1 | + | 25506 | 0.76 | 0.162857 |
Target: 5'- aCCUGGGCgGCGGGCCaacuagGCggguugagUUGGc -3' miRNA: 3'- -GGACCCGaCGCCCGGga----UGaaa-----AACC- -5' |
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31637 | 5' | -54.8 | NC_006879.1 | + | 33120 | 0.67 | 0.53337 |
Target: 5'- gCUGGuGUuuguggaagUGCGGGUCCagGCUcUUUUGGa -3' miRNA: 3'- gGACC-CG---------ACGCCCGGGa-UGA-AAAACC- -5' |
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31637 | 5' | -54.8 | NC_006879.1 | + | 19500 | 0.67 | 0.555556 |
Target: 5'- gCUGGGCUcCGGGuucgacCCCUACUUc---- -3' miRNA: 3'- gGACCCGAcGCCC------GGGAUGAAaaacc -5' |
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31637 | 5' | -54.8 | NC_006879.1 | + | 15113 | 0.66 | 0.576875 |
Target: 5'- gCCUggcGGGCagccugucgGCGGGCCCUcccggcgGCggcgUUGGc -3' miRNA: 3'- -GGA---CCCGa--------CGCCCGGGA-------UGaaa-AACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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