Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31638 | 5' | -53.2 | NC_006879.1 | + | 12753 | 0.67 | 0.651476 |
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Target: 5'- cCGACCACUGCuGgcggaagACagaCUGGGAAAGg -3' miRNA: 3'- -GCUGGUGACG-Cag-----UGg--GACCUUUUCa -5' |
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| 31638 | 5' | -53.2 | NC_006879.1 | + | 17561 | 0.67 | 0.651476 |
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Target: 5'- aGACCACUGCGgCAUCaucgaUGGGGuGGc -3' miRNA: 3'- gCUGGUGACGCaGUGGg----ACCUUuUCa -5' |
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| 31638 | 5' | -53.2 | NC_006879.1 | + | 23258 | 0.67 | 0.62835 |
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Target: 5'- gCGGCCguGCUG-GUCACCgUGGAGcuGg -3' miRNA: 3'- -GCUGG--UGACgCAGUGGgACCUUuuCa -5' |
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| 31638 | 5' | -53.2 | NC_006879.1 | + | 25111 | 0.71 | 0.410065 |
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Target: 5'- aGACCAgCUGCagcgCACCCUGGAc---- -3' miRNA: 3'- gCUGGU-GACGca--GUGGGACCUuuuca -5' |
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| 31638 | 5' | -53.2 | NC_006879.1 | + | 5476 | 0.79 | 0.116825 |
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Target: 5'- aCGACCugUGUcugcaCACCCUGGAAAAGg -3' miRNA: 3'- -GCUGGugACGca---GUGGGACCUUUUCa -5' |
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| 31638 | 5' | -53.2 | NC_006879.1 | + | 20598 | 1.07 | 0.001146 |
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Target: 5'- cCGACCACUGCGUCACCCUGGAAAAGUc -3' miRNA: 3'- -GCUGGUGACGCAGUGGGACCUUUUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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