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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31640 | 5' | -55.6 | NC_006879.1 | + | 21455 | 0.66 | 0.52291 |
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Target: 5'- aGUUACCcuaUCaugcaacgcgggcagCGACGCAUCCuGAUcCCUCu -3' miRNA: 3'- -CAGUGG---AG---------------GCUGCGUAGGuCUA-GGAG- -5' |
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| 31640 | 5' | -55.6 | NC_006879.1 | + | 9276 | 0.67 | 0.454908 |
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Target: 5'- aUCACCUCCcaacugcccgcgGACGCcccCCAGcggcccaccGUCCUCa -3' miRNA: 3'- cAGUGGAGG------------CUGCGua-GGUC---------UAGGAG- -5' |
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| 31640 | 5' | -55.6 | NC_006879.1 | + | 581 | 0.68 | 0.42471 |
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Target: 5'- uUCGCCUCCGuCGC-UUCAuGAUCUUUu -3' miRNA: 3'- cAGUGGAGGCuGCGuAGGU-CUAGGAG- -5' |
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| 31640 | 5' | -55.6 | NC_006879.1 | + | 13971 | 0.69 | 0.359158 |
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Target: 5'- uGUUACCUCCu-----UCCAGGUCCUCa -3' miRNA: 3'- -CAGUGGAGGcugcguAGGUCUAGGAG- -5' |
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| 31640 | 5' | -55.6 | NC_006879.1 | + | 22538 | 0.7 | 0.308066 |
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Target: 5'- cUCACUagCGuCGCGccggucaggccccUCCAGAUCCUCg -3' miRNA: 3'- cAGUGGagGCuGCGU-------------AGGUCUAGGAG- -5' |
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| 31640 | 5' | -55.6 | NC_006879.1 | + | 10704 | 0.73 | 0.201395 |
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Target: 5'- gGUCACCUCCucccuCGCgAUCaGGGUCCUCa -3' miRNA: 3'- -CAGUGGAGGcu---GCG-UAGgUCUAGGAG- -5' |
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| 31640 | 5' | -55.6 | NC_006879.1 | + | 21260 | 1.1 | 0.000414 |
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Target: 5'- gGUCACCUCCGACGCAUCCAGAUCCUCg -3' miRNA: 3'- -CAGUGGAGGCUGCGUAGGUCUAGGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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