miRNA: 3'- -CCGCCAagagCCAUGAaCACUGGGUg -5'
miRNA: 3'- -CCGCCAAGAGCCAUGAACACUGGGUG- -5'
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31653 | 5' | -53.4 | NC_006882.1 | + | 15552 | 0.66 | 0.757506 |
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Target: 5'- uGGUGGUaaa-GGUGCUgGUGGCUCAg -3' miRNA: 3'- -CCGCCAagagCCAUGAaCACUGGGUg -5' |
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| 31653 | 5' | -53.4 | NC_006882.1 | + | 18416 | 1.12 | 0.000781 |
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Target: 5'- aGGCGGUUCUCGGUACUUGUGACCCACu -3' miRNA: 3'- -CCGCCAAGAGCCAUGAACACUGGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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