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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31654 | 5' | -44 | NC_006882.1 | + | 17379 | 0.67 | 0.995302 |
Target: 5'- uGGGGA-GAAGAuGGUAGAAcAGCAAg -3' miRNA: 3'- cCUCCUaCUUCUcUCAUCUU-UUGUUa -5' |
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31654 | 5' | -44 | NC_006882.1 | + | 26533 | 0.69 | 0.983583 |
Target: 5'- cGGAGGA-GAAGAGAaUAGAuuAUGGg -3' miRNA: 3'- -CCUCCUaCUUCUCUcAUCUuuUGUUa -5' |
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31654 | 5' | -44 | NC_006882.1 | + | 6090 | 1.1 | 0.009675 |
Target: 5'- uGGAGGAUGAAGAGAGUAGAAAACAAUg -3' miRNA: 3'- -CCUCCUACUUCUCUCAUCUUUUGUUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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