Results 41 - 44 of 44 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31659 | 5' | -56.6 | NC_006883.1 | + | 23287 | 0.74 | 0.629179 |
Target: 5'- aCCUGGAGGACCuug---AGGACCaGGa -3' miRNA: 3'- -GGACCUCCUGGuggugaUCCUGGaCC- -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 26794 | 0.73 | 0.658379 |
Target: 5'- aCCUGGuGGACCagcaggACCAgUuGGACCUGc -3' miRNA: 3'- -GGACCuCCUGG------UGGUgAuCCUGGACc -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 21845 | 0.73 | 0.677762 |
Target: 5'- --aGGAccaGGACCACCACUcGGACCa-- -3' miRNA: 3'- ggaCCU---CCUGGUGGUGAuCCUGGacc -5' |
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31659 | 5' | -56.6 | NC_006883.1 | + | 213907 | 0.67 | 0.942784 |
Target: 5'- aCUGGuaAGGACCACCAUugUAGaGCCa-- -3' miRNA: 3'- gGACC--UCCUGGUGGUG--AUCcUGGacc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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