Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31661 | 3' | -57.8 | NC_006883.1 | + | 22061 | 0.74 | 0.51017 |
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Target: 5'- gACCACCAgCAGGaCCAGuuGGACCUg -3' miRNA: 3'- gUGGUGGUgGUCUaGGUCcuCCUGGA- -5' |
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| 31661 | 3' | -57.8 | NC_006883.1 | + | 21989 | 0.76 | 0.388042 |
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Target: 5'- gACCuCCACUuGGaCCAGGAGGACCUg -3' miRNA: 3'- gUGGuGGUGGuCUaGGUCCUCCUGGA- -5' |
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| 31661 | 3' | -57.8 | NC_006883.1 | + | 21959 | 0.67 | 0.860363 |
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Target: 5'- gACCAggagauCCAGuaCCAGGAGGACCa -3' miRNA: 3'- gUGGUggu---GGUCuaGGUCCUCCUGGa -5' |
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| 31661 | 3' | -57.8 | NC_006883.1 | + | 21881 | 0.74 | 0.500872 |
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Target: 5'- gACCACCAgCAGGaCCA-GAGGACCa -3' miRNA: 3'- gUGGUGGUgGUCUaGGUcCUCCUGGa -5' |
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| 31661 | 3' | -57.8 | NC_006883.1 | + | 21837 | 0.66 | 0.900817 |
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Target: 5'- gACCACCACuCGGA-CCAacaucaccuuGAGGGCCa -3' miRNA: 3'- gUGGUGGUG-GUCUaGGUc---------CUCCUGGa -5' |
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| 31661 | 3' | -57.8 | NC_006883.1 | + | 21790 | 0.78 | 0.341896 |
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Target: 5'- cCGCCACCuggACCAGAaggaCCuGGAGGACCa -3' miRNA: 3'- -GUGGUGG---UGGUCUa---GGuCCUCCUGGa -5' |
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| 31661 | 3' | -57.8 | NC_006883.1 | + | 14274 | 0.66 | 0.923561 |
|
Target: 5'- cCugCACCACCAGAUCCugcGGuu-GCUg -3' miRNA: 3'- -GugGUGGUGGUCUAGGu--CCuccUGGa -5' |
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| 31661 | 3' | -57.8 | NC_006883.1 | + | 12666 | 0.68 | 0.848379 |
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Target: 5'- cCACCGCCACCAGAagauuuagauugaccUCCuuGuGGAgCUg -3' miRNA: 3'- -GUGGUGGUGGUCU---------------AGGucCuCCUgGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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