Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31669 | 5' | -50.6 | NC_006883.1 | + | 22021 | 0.7 | 0.976562 |
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Target: 5'- -aCCcgUAGGACCAgCAGGACCaguUGGAc -3' miRNA: 3'- gaGGuaGUCUUGGUaGUCUUGG---ACCU- -5' |
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| 31669 | 5' | -50.6 | NC_006883.1 | + | 22087 | 0.7 | 0.978935 |
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Target: 5'- -aCCAcuuGGACCAggAGGACCUGGAg -3' miRNA: 3'- gaGGUaguCUUGGUagUCUUGGACCU- -5' |
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| 31669 | 5' | -50.6 | NC_006883.1 | + | 23349 | 0.7 | 0.981125 |
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Target: 5'- -cCUA-CAGGACCAguuGGACCUGGAg -3' miRNA: 3'- gaGGUaGUCUUGGUaguCUUGGACCU- -5' |
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| 31669 | 5' | -50.6 | NC_006883.1 | + | 28242 | 0.69 | 0.984989 |
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Target: 5'- uCUCCAccagUAGGACCAgCAGGACCagucGGAc -3' miRNA: 3'- -GAGGUa---GUCUUGGUaGUCUUGGa---CCU- -5' |
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| 31669 | 5' | -50.6 | NC_006883.1 | + | 21788 | 0.69 | 0.986678 |
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Target: 5'- -gCCAcCuGGACCAgaAGGACCUGGAg -3' miRNA: 3'- gaGGUaGuCUUGGUagUCUUGGACCU- -5' |
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| 31669 | 5' | -50.6 | NC_006883.1 | + | 220943 | 0.69 | 0.991352 |
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Target: 5'- cCUUCAUCAGAuuuccaccaaagauuACCAUCAGuaggauuACUUGGu -3' miRNA: 3'- -GAGGUAGUCU---------------UGGUAGUCu------UGGACCu -5' |
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| 31669 | 5' | -50.6 | NC_006883.1 | + | 27557 | 0.68 | 0.993046 |
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Target: 5'- -aCCAcCuGGACCAcCuGGACCUGGAg -3' miRNA: 3'- gaGGUaGuCUUGGUaGuCUUGGACCU- -5' |
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| 31669 | 5' | -50.6 | NC_006883.1 | + | 21815 | 0.66 | 0.999362 |
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Target: 5'- -aCCuUgAGGGCCAgCAGGACCaGGAc -3' miRNA: 3'- gaGGuAgUCUUGGUaGUCUUGGaCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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