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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31670 | 3' | -56 | NC_006883.1 | + | 23317 | 0.73 | 0.694863 |
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Target: 5'- aGGACCUuGAGGACCAgUUggaCCACCg-- -3' miRNA: 3'- -CCUGGAcCUCCUGGUgAA---GGUGGacu -5' |
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| 31670 | 3' | -56 | NC_006883.1 | + | 24969 | 0.73 | 0.714166 |
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Target: 5'- uGGACCUGauGGACCACUUggACCUGu -3' miRNA: 3'- -CCUGGACcuCCUGGUGAAggUGGACu -5' |
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| 31670 | 3' | -56 | NC_006883.1 | + | 28222 | 0.72 | 0.742613 |
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Target: 5'- aGGACCaGucGGACCAgUUCCACCa-- -3' miRNA: 3'- -CCUGGaCcuCCUGGUgAAGGUGGacu -5' |
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| 31670 | 3' | -56 | NC_006883.1 | + | 22209 | 0.72 | 0.755626 |
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Target: 5'- nGACCUGGAGGACCAgcaggaccaggaCCACCa-- -3' miRNA: 3'- cCUGGACCUCCUGGUgaa---------GGUGGacu -5' |
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| 31670 | 3' | -56 | NC_006883.1 | + | 23919 | 0.72 | 0.761144 |
|
Target: 5'- uGGACCUGuAGGACCuGCUggaCCugUUGAu -3' miRNA: 3'- -CCUGGACcUCCUGG-UGAa--GGugGACU- -5' |
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| 31670 | 3' | -56 | NC_006883.1 | + | 222980 | 0.71 | 0.77925 |
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Target: 5'- aGGACCaGGugaaccuuguGGACCAgUUCCACCa-- -3' miRNA: 3'- -CCUGGaCCu---------CCUGGUgAAGGUGGacu -5' |
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| 31670 | 3' | -56 | NC_006883.1 | + | 21581 | 0.66 | 0.965791 |
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Target: 5'- uGGuCCUGGuccuacuGGACCuGCUggUCCuCCUGGu -3' miRNA: 3'- -CCuGGACCu------CCUGG-UGA--AGGuGGACU- -5' |
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| 31670 | 3' | -56 | NC_006883.1 | + | 186560 | 0.67 | 0.939068 |
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Target: 5'- uGAUaCUGGAGaACCACcUgCACCUGAg -3' miRNA: 3'- cCUG-GACCUCcUGGUGaAgGUGGACU- -5' |
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| 31670 | 3' | -56 | NC_006883.1 | + | 211198 | 0.69 | 0.868481 |
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Target: 5'- uGGAgcuuauCCUGGAGGACCACaagCC-UUUGAa -3' miRNA: 3'- -CCU------GGACCUCCUGGUGaa-GGuGGACU- -5' |
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| 31670 | 3' | -56 | NC_006883.1 | + | 23398 | 0.72 | 0.761144 |
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Target: 5'- uGGugCUGGugguGGACCuggUCCugCUGGu -3' miRNA: 3'- -CCugGACCu---CCUGGugaAGGugGACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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