Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31673 | 3' | -47.2 | NC_006883.1 | + | 179988 | 0.66 | 0.999981 |
Target: 5'- cAUUGGUAGGUUGAauccaUUCUuuaaAUUCUUGuCGa -3' miRNA: 3'- -UGACCAUUCAGCU-----AAGA----UGAGAAC-GC- -5' |
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31673 | 3' | -47.2 | NC_006883.1 | + | 206410 | 0.67 | 0.999954 |
Target: 5'- --aGGUGAGaUgGAcaCUACUCUUGCu -3' miRNA: 3'- ugaCCAUUC-AgCUaaGAUGAGAACGc -5' |
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31673 | 3' | -47.2 | NC_006883.1 | + | 235932 | 0.68 | 0.999863 |
Target: 5'- uGCUGGUGguGGUgcuucUGGUUCUGCagaaUCUUGUGc -3' miRNA: 3'- -UGACCAU--UCA-----GCUAAGAUG----AGAACGC- -5' |
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31673 | 3' | -47.2 | NC_006883.1 | + | 145066 | 1.11 | 0.026636 |
Target: 5'- gACUGGUAAGUCGAUUCUACUCUUGCGa -3' miRNA: 3'- -UGACCAUUCAGCUAAGAUGAGAACGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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