Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31674 | 3' | -42.3 | NC_006883.1 | + | 21556 | 0.68 | 1 |
Target: 5'- cUCAAGGUGAu--GUugGUCCGaguGGUGg -3' miRNA: 3'- -AGUUUUAUUuuuCGugCAGGU---CCAUa -5' |
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31674 | 3' | -42.3 | NC_006883.1 | + | 39416 | 0.67 | 1 |
Target: 5'- cUCAAGAU-GAAGGUGa-UCCAGGUAa -3' miRNA: 3'- -AGUUUUAuUUUUCGUgcAGGUCCAUa -5' |
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31674 | 3' | -42.3 | NC_006883.1 | + | 146052 | 0.67 | 1 |
Target: 5'- aUCAAAGUGGucauggagaauauguAAAGgGCGUuuGGGUAUc -3' miRNA: 3'- -AGUUUUAUU---------------UUUCgUGCAggUCCAUA- -5' |
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31674 | 3' | -42.3 | NC_006883.1 | + | 28214 | 0.66 | 1 |
Target: 5'- -gGAGAUGGuccucCugGUCCAGGUGg -3' miRNA: 3'- agUUUUAUUuuuc-GugCAGGUCCAUa -5' |
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31674 | 3' | -42.3 | NC_006883.1 | + | 44561 | 0.66 | 1 |
Target: 5'- aUUAAAAUAAu--GCACGUUCAuaguGGUGa -3' miRNA: 3'- -AGUUUUAUUuuuCGUGCAGGU----CCAUa -5' |
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31674 | 3' | -42.3 | NC_006883.1 | + | 17012 | 0.66 | 1 |
Target: 5'- aCGAcaccuGGAGCACGuuUCCAGGUu- -3' miRNA: 3'- aGUUuuauuUUUCGUGC--AGGUCCAua -5' |
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31674 | 3' | -42.3 | NC_006883.1 | + | 235109 | 0.67 | 1 |
Target: 5'- cCAGAAUGAAAAuGU---UCCAGGUAUa -3' miRNA: 3'- aGUUUUAUUUUU-CGugcAGGUCCAUA- -5' |
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31674 | 3' | -42.3 | NC_006883.1 | + | 123280 | 0.67 | 1 |
Target: 5'- cCAuAAUuuAAAGCAgGUCUAGGa-- -3' miRNA: 3'- aGUuUUAuuUUUCGUgCAGGUCCaua -5' |
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31674 | 3' | -42.3 | NC_006883.1 | + | 237427 | 0.72 | 0.999988 |
Target: 5'- -uGAAGUAAAAGGCAC-UUUAGGUAa -3' miRNA: 3'- agUUUUAUUUUUCGUGcAGGUCCAUa -5' |
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31674 | 3' | -42.3 | NC_006883.1 | + | 24911 | 0.72 | 0.999984 |
Target: 5'- gCAGAuuuuGGAGCACuUCCAGGUGc -3' miRNA: 3'- aGUUUuauuUUUCGUGcAGGUCCAUa -5' |
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31674 | 3' | -42.3 | NC_006883.1 | + | 174553 | 0.82 | 0.946178 |
Target: 5'- aUCAGAuaGAAAGGCugGUUCAGGUGg -3' miRNA: 3'- -AGUUUuaUUUUUCGugCAGGUCCAUa -5' |
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31674 | 3' | -42.3 | NC_006883.1 | + | 146383 | 1.08 | 0.112352 |
Target: 5'- uUCAAAAUAAAAAGCACGUCCAGGUAUc -3' miRNA: 3'- -AGUUUUAUUUUUCGUGCAGGUCCAUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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