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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31676 | 3' | -47.3 | NC_006883.1 | + | 92207 | 0.66 | 0.999982 |
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Target: 5'- gUGaCUUUaGAGCAGAAauuACCUGCGa -3' miRNA: 3'- gACgGAAAcCUCGUUUUau-UGGACGU- -5' |
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| 31676 | 3' | -47.3 | NC_006883.1 | + | 96087 | 0.66 | 0.999966 |
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Target: 5'- uCUGCUgaUGGAGCAAcuuuagGAUuagcACCUGUu -3' miRNA: 3'- -GACGGaaACCUCGUU------UUAu---UGGACGu -5' |
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| 31676 | 3' | -47.3 | NC_006883.1 | + | 164627 | 0.67 | 0.99992 |
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Target: 5'- uCUGCUauuaaugUGGcaguAGCAccaccaaguGAAUGACCUGCAa -3' miRNA: 3'- -GACGGaa-----ACC----UCGU---------UUUAUUGGACGU- -5' |
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| 31676 | 3' | -47.3 | NC_006883.1 | + | 80264 | 0.67 | 0.999894 |
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Target: 5'- gUGCCUcuagaUGGGGUuccgauACCUGCAu -3' miRNA: 3'- gACGGAa----ACCUCGuuuuauUGGACGU- -5' |
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| 31676 | 3' | -47.3 | NC_006883.1 | + | 226165 | 0.68 | 0.999634 |
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Target: 5'- aCUGCCgUUGGAGCAAGugcauUAGCUa--- -3' miRNA: 3'- -GACGGaAACCUCGUUUu----AUUGGacgu -5' |
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| 31676 | 3' | -47.3 | NC_006883.1 | + | 48102 | 0.69 | 0.998421 |
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Target: 5'- aCUGCCUuuucguaguUUGGGGCAAGAUAcAUgUGUu -3' miRNA: 3'- -GACGGA---------AACCUCGUUUUAU-UGgACGu -5' |
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| 31676 | 3' | -47.3 | NC_006883.1 | + | 119988 | 0.7 | 0.9976 |
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Target: 5'- aUGCCguuaacaaaagcaucUGGAGCAGAAgGAUCUGCc -3' miRNA: 3'- gACGGaa-------------ACCUCGUUUUaUUGGACGu -5' |
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| 31676 | 3' | -47.3 | NC_006883.1 | + | 183934 | 1.09 | 0.02561 |
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Target: 5'- uCUGCCUUUGGAGCAAAAUAACCUGCAc -3' miRNA: 3'- -GACGGAAACCUCGUUUUAUUGGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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