Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31676 | 5' | -44.5 | NC_006883.1 | + | 67857 | 0.66 | 1 |
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Target: 5'- -aUGCAGGUUAgcgAGCguaCCAU-GAGGCg -3' miRNA: 3'- ucAUGUCCGAUa--UUGa--GGUAuUUCUG- -5' |
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| 31676 | 5' | -44.5 | NC_006883.1 | + | 67897 | 0.66 | 1 |
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Target: 5'- aAGUcuCAGGaauuuuuuuuCUCCAUAAGGACa -3' miRNA: 3'- -UCAu-GUCCgauauu----GAGGUAUUUCUG- -5' |
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| 31676 | 5' | -44.5 | NC_006883.1 | + | 32395 | 0.68 | 0.999997 |
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Target: 5'- aGGUuCAGGCUaccauuuGUAugUCCAaUAAGGAa -3' miRNA: 3'- -UCAuGUCCGA-------UAUugAGGU-AUUUCUg -5' |
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| 31676 | 5' | -44.5 | NC_006883.1 | + | 166643 | 0.73 | 0.998128 |
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Target: 5'- -cUACAGGac--AACUCCGUGAAGAUg -3' miRNA: 3'- ucAUGUCCgauaUUGAGGUAUUUCUG- -5' |
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| 31676 | 5' | -44.5 | NC_006883.1 | + | 183653 | 0.83 | 0.844804 |
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Target: 5'- uGGUGCAGGUUAUuuuGCUCCA--AAGGCa -3' miRNA: 3'- -UCAUGUCCGAUAu--UGAGGUauUUCUG- -5' |
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| 31676 | 5' | -44.5 | NC_006883.1 | + | 183900 | 0.97 | 0.243825 |
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Target: 5'- aAGUACAGGCUAUAACUCCuaAAAGACu -3' miRNA: 3'- -UCAUGUCCGAUAUUGAGGuaUUUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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