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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31683 | 3' | -55.4 | NC_006883.1 | + | 162720 | 0.7 | 0.875 |
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Target: 5'- uCUGGCAGGUgCUcCAGGAUCAGCa-- -3' miRNA: 3'- -GGCUGUCCAgGGuGUCCUGGUUGacc -5' |
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| 31683 | 3' | -55.4 | NC_006883.1 | + | 21860 | 0.7 | 0.881811 |
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Target: 5'- aCCaGCAGGUCCaguaggacCAGGACCAccACUcGGa -3' miRNA: 3'- -GGcUGUCCAGGgu------GUCCUGGU--UGA-CC- -5' |
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| 31683 | 3' | -55.4 | NC_006883.1 | + | 22252 | 0.7 | 0.88842 |
|
Target: 5'- aCCaGCAGaUCCaGCAGGACCAGgaGGa -3' miRNA: 3'- -GGcUGUCcAGGgUGUCCUGGUUgaCC- -5' |
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| 31683 | 3' | -55.4 | NC_006883.1 | + | 23176 | 0.69 | 0.920471 |
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Target: 5'- aCCaGCAGGUCCaguUaaaccuggagcaccaGCAGGACCAGUUGGa -3' miRNA: 3'- -GGcUGUCCAGG---G---------------UGUCCUGGUUGACC- -5' |
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| 31683 | 3' | -55.4 | NC_006883.1 | + | 28183 | 0.68 | 0.947034 |
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Target: 5'- uCCGuCAGcaCCaGCAGGACCAGUUGGa -3' miRNA: 3'- -GGCuGUCcaGGgUGUCCUGGUUGACC- -5' |
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| 31683 | 3' | -55.4 | NC_006883.1 | + | 222950 | 0.67 | 0.958501 |
|
Target: 5'- aCCaGCAGGaCCaGCAGGACCAGgaGa -3' miRNA: 3'- -GGcUGUCCaGGgUGUCCUGGUUgaCc -5' |
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| 31683 | 3' | -55.4 | NC_006883.1 | + | 23725 | 0.67 | 0.970963 |
|
Target: 5'- aCCuGCuGGaCCUGgAGGACCuGCUGGa -3' miRNA: 3'- -GGcUGuCCaGGGUgUCCUGGuUGACC- -5' |
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| 31683 | 3' | -55.4 | NC_006883.1 | + | 22143 | 0.66 | 0.973604 |
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Target: 5'- -gGAcCAGGUgaaCCACuuGGACCAGCaGGa -3' miRNA: 3'- ggCU-GUCCAg--GGUGu-CCUGGUUGaCC- -5' |
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| 31683 | 3' | -55.4 | NC_006883.1 | + | 22069 | 0.66 | 0.984257 |
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Target: 5'- aCCuGgAGGaCCaccaGCAGGACCAGUUGGa -3' miRNA: 3'- -GGcUgUCCaGGg---UGUCCUGGUUGACC- -5' |
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| 31683 | 3' | -55.4 | NC_006883.1 | + | 23323 | 0.75 | 0.627553 |
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Target: 5'- aCCaGCAGGaCCUugAGGACCAGUUGGa -3' miRNA: 3'- -GGcUGUCCaGGGugUCCUGGUUGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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