Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31683 | 5' | -54.7 | NC_006883.1 | + | 21744 | 0.67 | 0.946506 |
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Target: 5'- uUCCAGUUGGaCCUGgaGGAccaGCAGGAc -3' miRNA: 3'- cAGGUUGACCaGGACgaCCU---CGUUCU- -5' |
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| 31683 | 5' | -54.7 | NC_006883.1 | + | 26714 | 0.67 | 0.950705 |
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Target: 5'- uUCCAGgaGGaCCUGCUGGAcCuGGAg -3' miRNA: 3'- cAGGUUgaCCaGGACGACCUcGuUCU- -5' |
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| 31683 | 5' | -54.7 | NC_006883.1 | + | 22216 | 0.67 | 0.96196 |
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Target: 5'- -aCCAuCUGGaCCUGgaGGAccaGCAGGAc -3' miRNA: 3'- caGGUuGACCaGGACgaCCU---CGUUCU- -5' |
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| 31683 | 5' | -54.7 | NC_006883.1 | + | 23410 | 0.66 | 0.965278 |
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Target: 5'- -aCCAuCUGGUCCaGUUGGAccuGUggGAc -3' miRNA: 3'- caGGUuGACCAGGaCGACCU---CGuuCU- -5' |
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| 31683 | 5' | -54.7 | NC_006883.1 | + | 134593 | 0.66 | 0.974002 |
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Target: 5'- uUCUAcuACUGGUUCUGgUGGuGUAGGu -3' miRNA: 3'- cAGGU--UGACCAGGACgACCuCGUUCu -5' |
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| 31683 | 5' | -54.7 | NC_006883.1 | + | 28366 | 0.73 | 0.70276 |
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Target: 5'- -aCCAGgaGGUCCUGgaGGAccaGCAGGAc -3' miRNA: 3'- caGGUUgaCCAGGACgaCCU---CGUUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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