Results 61 - 66 of 66 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31684 | 5' | -53.6 | NC_006883.1 | + | 21695 | 0.74 | 0.791228 |
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Target: 5'- uGGAucucCUGGUCCuGCaGGUCCUcCUGGu -3' miRNA: 3'- -CCUu---GACCAGGuUGaCCAGGAcGACC- -5' |
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| 31684 | 5' | -53.6 | NC_006883.1 | + | 21667 | 0.82 | 0.385417 |
|
Target: 5'- -cAACaGGUCCAACUGGUCCUcCUGGu -3' miRNA: 3'- ccUUGaCCAGGUUGACCAGGAcGACC- -5' |
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| 31684 | 5' | -53.6 | NC_006883.1 | + | 21596 | 0.95 | 0.070049 |
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Target: 5'- uGGAccuGCUGGUCCucCUGGUCCUGCUGGu -3' miRNA: 3'- -CCU---UGACCAGGuuGACCAGGACGACC- -5' |
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| 31684 | 5' | -53.6 | NC_006883.1 | + | 21515 | 0.85 | 0.246231 |
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Target: 5'- aGGuccuuCUGGUCCAGguggcggucCUGGUCCUGCUGGc -3' miRNA: 3'- -CCuu---GACCAGGUU---------GACCAGGACGACC- -5' |
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| 31684 | 5' | -53.6 | NC_006883.1 | + | 21484 | 0.72 | 0.857299 |
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Target: 5'- -cAACUGGaaUAACUGGUCCUuCUGGu -3' miRNA: 3'- ccUUGACCagGUUGACCAGGAcGACC- -5' |
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| 31684 | 5' | -53.6 | NC_006883.1 | + | 21446 | 0.9 | 0.1369 |
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Target: 5'- cGGuGgUGGUCCuACUGGUCCUGCUGGu -3' miRNA: 3'- -CCuUgACCAGGuUGACCAGGACGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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