miRNA: 3'- uuuUCUGCAUCUACAUaaCCc--ACCAC- -5'
miRNA: 3'- -UUUUCU-GCAUCUACauaACC-CACCAc -5'
miRNA: 3'- -UUUUCugCA--UCUACa-----UAACCC-ACCAc -5'
miRNA: 3'- uuUUCUGCau---CUACAUa-ACCCACCAC- -5'
miRNA: 3'- -UUUUCUGCAUCUACAUAACCCACCAC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31687 | 3' | -46.7 | NC_006883.1 | + | 110001 | 0.67 | 0.999984 |
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Target: 5'- --uAGGCGUAGAUGUA--GGuuuUGGUGu -3' miRNA: 3'- uuuUCUGCAUCUACAUaaCCc--ACCAC- -5' |
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| 31687 | 3' | -46.7 | NC_006883.1 | + | 195936 | 0.67 | 0.999984 |
|
Target: 5'- aAGGAGAgCGUGGAUGaaaUGGuGUGGUu -3' miRNA: 3'- -UUUUCU-GCAUCUACauaACC-CACCAc -5' |
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| 31687 | 3' | -46.7 | NC_006883.1 | + | 39120 | 0.68 | 0.999795 |
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Target: 5'- gAAGAGugGUucAGGUGgaauugAUUGGGaUGGUa -3' miRNA: 3'- -UUUUCugCA--UCUACa-----UAACCC-ACCAc -5' |
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| 31687 | 3' | -46.7 | NC_006883.1 | + | 192735 | 0.71 | 0.997488 |
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Target: 5'- -cAAGAUGguauuGAUGUGcaUGGGUGGUGc -3' miRNA: 3'- uuUUCUGCau---CUACAUa-ACCCACCAC- -5' |
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| 31687 | 3' | -46.7 | NC_006883.1 | + | 109691 | 1.06 | 0.049242 |
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Target: 5'- cAAAAGACGUAGAUGUAUUGGGUGGUGc -3' miRNA: 3'- -UUUUCUGCAUCUACAUAACCCACCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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