miRNA: 3'- -ACUACUGCUACCACCAUUUCCUUAGU- -5'
miRNA: 3'- aCUACU---GCUA---CCACCAUUUCC-UUAgu -5'
miRNA: 3'- aCUACUGCU------ACCACCAUUUCCuUAGu -5'
miRNA: 3'- -ACUACUGCUACCACCauuuccuuagu -5'
miRNA: 3'- aCUACuGCUACCACCAU------UUCCUuagu -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31688 | 3' | -46.6 | NC_006883.1 | + | 208677 | 1.11 | 0.029824 |
|
Target: 5'- aUGAUGACGAUGGUGGUAAAGGAAUCAg -3' miRNA: 3'- -ACUACUGCUACCACCAUUUCCUUAGU- -5' |
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| 31688 | 3' | -46.6 | NC_006883.1 | + | 212487 | 0.66 | 0.999998 |
|
Target: 5'- aGAUGAuacUGGUagaGGUGGUAAGGGuAAUa- -3' miRNA: 3'- aCUACU---GCUA---CCACCAUUUCC-UUAgu -5' |
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| 31688 | 3' | -46.6 | NC_006883.1 | + | 215428 | 0.81 | 0.82965 |
|
Target: 5'- aGAUGAUGAuacgucUGGUGGUGAAGG-AUCu -3' miRNA: 3'- aCUACUGCU------ACCACCAUUUCCuUAGu -5' |
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| 31688 | 3' | -46.6 | NC_006883.1 | + | 234137 | 0.66 | 0.999993 |
|
Target: 5'- gUGGUGGCGGUGGUGGa---------- -3' miRNA: 3'- -ACUACUGCUACCACCauuuccuuagu -5' |
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| 31688 | 3' | -46.6 | NC_006883.1 | + | 236175 | 0.67 | 0.999982 |
|
Target: 5'- gGGUGcUGGUGGUGGUGcaaauaGAGGAc--- -3' miRNA: 3'- aCUACuGCUACCACCAU------UUCCUuagu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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