miRNA: 3'- aCUACUGCUACCACCAU-UUCcuUAGU- -5'
miRNA: 3'- acuACugCUACCACCAUuuCCUUAGu -5'
miRNA: 3'- aCUaCUG--CUACCACCAUUuCCUUAGu -5'
miRNA: 3'- -ACUACUGCUACCACCAUuuccuuagu -5'
miRNA: 3'- -ACUACUg--CU--ACCACCAUu---UCCUUagu -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31688 | 3' | -46.6 | NC_006883.1 | + | 25065 | 0.76 | 0.964529 |
|
Target: 5'- gGGUGAUGGUGGUGGUGuAAGu-AUCAu -3' miRNA: 3'- aCUACUGCUACCACCAU-UUCcuUAGU- -5' |
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| 31688 | 3' | -46.6 | NC_006883.1 | + | 18176 | 0.71 | 0.998163 |
|
Target: 5'- --uUGuuGAUGGUGGUGuuGGAAUUg -3' miRNA: 3'- acuACugCUACCACCAUuuCCUUAGu -5' |
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| 31688 | 3' | -46.6 | NC_006883.1 | + | 16280 | 0.73 | 0.993268 |
|
Target: 5'- aGA-GACcuGAUGGUGGUAAuGGAGUUg -3' miRNA: 3'- aCUaCUG--CUACCACCAUUuCCUUAGu -5' |
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| 31688 | 3' | -46.6 | NC_006883.1 | + | 12402 | 0.71 | 0.998163 |
|
Target: 5'- cUGGUGGCGGUGGUGGUGc-------- -3' miRNA: 3'- -ACUACUGCUACCACCAUuuccuuagu -5' |
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| 31688 | 3' | -46.6 | NC_006883.1 | + | 12044 | 0.67 | 0.999987 |
|
Target: 5'- aUGAUGAaggGAuuUGGUGGUAucauAGGAAc-- -3' miRNA: 3'- -ACUACUg--CU--ACCACCAUu---UCCUUagu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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