miRNA: 3'- -ACUACUGCUACC---ACCAUUUCCuu-AGu -5'
miRNA: 3'- aCUACUGCUACCACCAU-UUCcuUAGU- -5'
miRNA: 3'- -ACUACUGCUACCaCCAU------UUCCUUA-GU- -5'
miRNA: 3'- aCUACUGCU------ACCACCAUUUCCuUAGu -5'
miRNA: 3'- -ACUACUGCUACCACCAUUUCCUUAGU- -5'
Results 21 - 25 of 25 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31688 | 3' | -46.6 | NC_006883.1 | + | 111878 | 0.74 | 0.989809 |
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Target: 5'- uUGGUGAUGGUGGagcUGGUGGAGGuacUCc -3' miRNA: 3'- -ACUACUGCUACC---ACCAUUUCCuu-AGu -5' |
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| 31688 | 3' | -46.6 | NC_006883.1 | + | 25065 | 0.76 | 0.964529 |
|
Target: 5'- gGGUGAUGGUGGUGGUGuAAGu-AUCAu -3' miRNA: 3'- aCUACUGCUACCACCAU-UUCcuUAGU- -5' |
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| 31688 | 3' | -46.6 | NC_006883.1 | + | 39521 | 0.76 | 0.960996 |
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Target: 5'- aUGGUGGCGGUGGcGGUGgagaacAGGGAAUaCAa -3' miRNA: 3'- -ACUACUGCUACCaCCAU------UUCCUUA-GU- -5' |
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| 31688 | 3' | -46.6 | NC_006883.1 | + | 215428 | 0.81 | 0.82965 |
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Target: 5'- aGAUGAUGAuacgucUGGUGGUGAAGG-AUCu -3' miRNA: 3'- aCUACUGCU------ACCACCAUUUCCuUAGu -5' |
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| 31688 | 3' | -46.6 | NC_006883.1 | + | 208677 | 1.11 | 0.029824 |
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Target: 5'- aUGAUGACGAUGGUGGUAAAGGAAUCAg -3' miRNA: 3'- -ACUACUGCUACCACCAUUUCCUUAGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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