Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31698 | 3' | -46.1 | NC_006934.1 | + | 3315 | 0.66 | 0.695232 |
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Target: 5'- -cAGAUgcgGAGGAAGGAGAauCAGGGc-- -3' miRNA: 3'- gaUUUAa--CUCCUUUCUCU--GUCCUccc -5' |
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| 31698 | 3' | -46.1 | NC_006934.1 | + | 3220 | 0.67 | 0.654938 |
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Target: 5'- aUAGGcUGAGGAGgguguauggguuAGGGACAGuuGGGu -3' miRNA: 3'- gAUUUaACUCCUU------------UCUCUGUCcuCCC- -5' |
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| 31698 | 3' | -46.1 | NC_006934.1 | + | 6197 | 0.67 | 0.610298 |
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Target: 5'- -gGAGUUGGGGcuGGGGAgGGGGauuuagacaacaguGGGa -3' miRNA: 3'- gaUUUAACUCCuuUCUCUgUCCU--------------CCC- -5' |
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| 31698 | 3' | -46.1 | NC_006934.1 | + | 941 | 0.69 | 0.495524 |
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Target: 5'- --------cGGAGAGGGAgAGGAGGa -3' miRNA: 3'- gauuuaacuCCUUUCUCUgUCCUCCc -5' |
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| 31698 | 3' | -46.1 | NC_006934.1 | + | 2 | 0.7 | 0.470431 |
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Target: 5'- -----aUGuGGGAGGGAGcucCGGGGGGGg -3' miRNA: 3'- gauuuaAC-UCCUUUCUCu--GUCCUCCC- -5' |
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| 31698 | 3' | -46.1 | NC_006934.1 | + | 1762 | 0.72 | 0.355966 |
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Target: 5'- -gAGAgggUGAGGAcagaAAGuAGGCGGGAGGu -3' miRNA: 3'- gaUUUa--ACUCCU----UUC-UCUGUCCUCCc -5' |
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| 31698 | 3' | -46.1 | NC_006934.1 | + | 1013 | 0.72 | 0.345647 |
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Target: 5'- ------aGAGGGcGGGAGACuauGGGGGGg -3' miRNA: 3'- gauuuaaCUCCU-UUCUCUGu--CCUCCC- -5' |
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| 31698 | 3' | -46.1 | NC_006934.1 | + | 1434 | 0.72 | 0.335545 |
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Target: 5'- ------aGAGGAA--GGugAGGAGGGg -3' miRNA: 3'- gauuuaaCUCCUUucUCugUCCUCCC- -5' |
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| 31698 | 3' | -46.1 | NC_006934.1 | + | 7737 | 0.73 | 0.306544 |
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Target: 5'- -gGAAUguGGGAGGGAGcucCGGGGGGGg -3' miRNA: 3'- gaUUUAacUCCUUUCUCu--GUCCUCCC- -5' |
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| 31698 | 3' | -46.1 | NC_006934.1 | + | 5499 | 1.14 | 0.000376 |
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Target: 5'- gCUAAAUUGAGGAAAGAGACAGGAGGGg -3' miRNA: 3'- -GAUUUAACUCCUUUCUCUGUCCUCCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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