Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31713 | 3' | -58.7 | NC_006938.1 | + | 59315 | 0.66 | 0.595563 |
Target: 5'- aCGG-CAGGuaUCCGGCCGUGAucugguaGAGg -3' miRNA: 3'- cGCCgGUCUucAGGCCGGCACU-------CUCa -5' |
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31713 | 3' | -58.7 | NC_006938.1 | + | 27824 | 0.66 | 0.563958 |
Target: 5'- gGCGGCCGGAagggucaGGUCCaGCUG-GAcGGGc -3' miRNA: 3'- -CGCCGGUCU-------UCAGGcCGGCaCU-CUCa -5' |
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31713 | 3' | -58.7 | NC_006938.1 | + | 52411 | 0.66 | 0.554558 |
Target: 5'- cGgGGCCAGGgcGGUggcaacaaCCGGCCcGUGAcAGUg -3' miRNA: 3'- -CgCCGGUCU--UCA--------GGCCGG-CACUcUCA- -5' |
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31713 | 3' | -58.7 | NC_006938.1 | + | 37065 | 0.67 | 0.533843 |
Target: 5'- cGUGGgaCCAGAGGccCCGGCagCGUGAGAc- -3' miRNA: 3'- -CGCC--GGUCUUCa-GGCCG--GCACUCUca -5' |
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31713 | 3' | -58.7 | NC_006938.1 | + | 12049 | 0.67 | 0.523588 |
Target: 5'- cCGGCCGGAAGUCC-GCCcUGAa--- -3' miRNA: 3'- cGCCGGUCUUCAGGcCGGcACUcuca -5' |
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31713 | 3' | -58.7 | NC_006938.1 | + | 60379 | 0.67 | 0.513409 |
Target: 5'- aGUGGCCAGcuGGaCCGGCaccaacGAGAGUg -3' miRNA: 3'- -CGCCGGUCu-UCaGGCCGgca---CUCUCA- -5' |
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31713 | 3' | -58.7 | NC_006938.1 | + | 31615 | 0.67 | 0.493303 |
Target: 5'- aGCGGaCCaAGAAGUucauggacaagCCGGUCGUG-GAGc -3' miRNA: 3'- -CGCC-GG-UCUUCA-----------GGCCGGCACuCUCa -5' |
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31713 | 3' | -58.7 | NC_006938.1 | + | 58652 | 0.68 | 0.42606 |
Target: 5'- cGCGGCCGGAGacacaauuGUCCaGGCgGUGAcuGGUc -3' miRNA: 3'- -CGCCGGUCUU--------CAGG-CCGgCACUc-UCA- -5' |
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31713 | 3' | -58.7 | NC_006938.1 | + | 15827 | 0.69 | 0.389294 |
Target: 5'- -aGGCCAGAGGUCUGGaucucguCCGUGGcaaccgcgcuGAGg -3' miRNA: 3'- cgCCGGUCUUCAGGCC-------GGCACU----------CUCa -5' |
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31713 | 3' | -58.7 | NC_006938.1 | + | 34284 | 0.7 | 0.372975 |
Target: 5'- -gGGCCAGGGcGUCUacuCCGUGGGAGUg -3' miRNA: 3'- cgCCGGUCUU-CAGGcc-GGCACUCUCA- -5' |
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31713 | 3' | -58.7 | NC_006938.1 | + | 21114 | 0.71 | 0.302173 |
Target: 5'- aCGGCaAGGGuUCCGGCCGaGGGAGUg -3' miRNA: 3'- cGCCGgUCUUcAGGCCGGCaCUCUCA- -5' |
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31713 | 3' | -58.7 | NC_006938.1 | + | 42481 | 0.72 | 0.25397 |
Target: 5'- uGgGGCCGGAAGguggucgucgugaUCCGGCCGUucGGGa -3' miRNA: 3'- -CgCCGGUCUUC-------------AGGCCGGCAcuCUCa -5' |
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31713 | 3' | -58.7 | NC_006938.1 | + | 62347 | 1.09 | 0.000568 |
Target: 5'- gGCGGCCAGAAGUCCGGCCGUGAGAGUg -3' miRNA: 3'- -CGCCGGUCUUCAGGCCGGCACUCUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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