Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31714 | 3' | -58.4 | NC_006938.1 | + | 7178 | 0.67 | 0.545056 |
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Target: 5'- uGGCCGaGCUCacaagagagCGCGUCcGCGUUGAc -3' miRNA: 3'- cUUGGC-CGGGga-------GCGCAGuCGCAACU- -5' |
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| 31714 | 3' | -58.4 | NC_006938.1 | + | 13521 | 0.68 | 0.474165 |
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Target: 5'- cGGGCacaaaGGCCUCUCGCGUUccagaGGCGggGc -3' miRNA: 3'- -CUUGg----CCGGGGAGCGCAG-----UCGCaaCu -5' |
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| 31714 | 3' | -58.4 | NC_006938.1 | + | 31705 | 0.69 | 0.426465 |
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Target: 5'- aGACCGGCCgCaagggCGCGUCAGCc---- -3' miRNA: 3'- cUUGGCCGGgGa----GCGCAGUCGcaacu -5' |
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| 31714 | 3' | -58.4 | NC_006938.1 | + | 36537 | 0.71 | 0.302112 |
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Target: 5'- cGACCuGGCCCgUCGCGagacgUCGGCGUUc- -3' miRNA: 3'- cUUGG-CCGGGgAGCGC-----AGUCGCAAcu -5' |
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| 31714 | 3' | -58.4 | NC_006938.1 | + | 12658 | 0.78 | 0.109489 |
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Target: 5'- --cUCGGCCgCCUCGCGgaaGGCGUUGAg -3' miRNA: 3'- cuuGGCCGG-GGAGCGCag-UCGCAACU- -5' |
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| 31714 | 3' | -58.4 | NC_006938.1 | + | 61509 | 1.09 | 0.000642 |
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Target: 5'- aGAACCGGCCCCUCGCGUCAGCGUUGAg -3' miRNA: 3'- -CUUGGCCGGGGAGCGCAGUCGCAACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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