Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31715 | 5' | -59.2 | NC_006938.1 | + | 50769 | 0.66 | 0.54786 |
Target: 5'- cUGCgcGGcgaCGAGCUCCgcUCCgUCCUGGa- -3' miRNA: 3'- -ACGa-CCa--GCUCGAGG--AGG-AGGACCac -5' |
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31715 | 5' | -59.2 | NC_006938.1 | + | 59503 | 0.66 | 0.527101 |
Target: 5'- gGgaGGUCGAGCgcgUCCUUCUUCaGGa- -3' miRNA: 3'- aCgaCCAGCUCG---AGGAGGAGGaCCac -5' |
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31715 | 5' | -59.2 | NC_006938.1 | + | 61709 | 0.66 | 0.506644 |
Target: 5'- cGCUGaaUGgaaccGGCUcCCUCCUCCUGGa- -3' miRNA: 3'- aCGACcaGC-----UCGA-GGAGGAGGACCac -5' |
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31715 | 5' | -59.2 | NC_006938.1 | + | 11257 | 0.66 | 0.506644 |
Target: 5'- cUGCUGGUCGAcCUCCgugagaUCUUGGa- -3' miRNA: 3'- -ACGACCAGCUcGAGGagg---AGGACCac -5' |
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31715 | 5' | -59.2 | NC_006938.1 | + | 62099 | 0.68 | 0.392397 |
Target: 5'- cGCccucgGGagGcAGCUCCUUCUCCUgcGGUGg -3' miRNA: 3'- aCGa----CCagC-UCGAGGAGGAGGA--CCAC- -5' |
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31715 | 5' | -59.2 | NC_006938.1 | + | 35070 | 0.69 | 0.34998 |
Target: 5'- uUGCcacGGagGAGCUCCUCCUuCUUGGc- -3' miRNA: 3'- -ACGa--CCagCUCGAGGAGGA-GGACCac -5' |
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31715 | 5' | -59.2 | NC_006938.1 | + | 28513 | 0.69 | 0.34998 |
Target: 5'- aUGCcgaGGUCcagggaGGCgUCCUCCUCCUGGa- -3' miRNA: 3'- -ACGa--CCAGc-----UCG-AGGAGGAGGACCac -5' |
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31715 | 5' | -59.2 | NC_006938.1 | + | 45612 | 0.7 | 0.289133 |
Target: 5'- cUGUUGcUCGAuCUCCUUCUCCUGGa- -3' miRNA: 3'- -ACGACcAGCUcGAGGAGGAGGACCac -5' |
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31715 | 5' | -59.2 | NC_006938.1 | + | 4857 | 0.72 | 0.230966 |
Target: 5'- aGgUGGUCGAGCUCCUgUCUCCgacGGc- -3' miRNA: 3'- aCgACCAGCUCGAGGA-GGAGGa--CCac -5' |
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31715 | 5' | -59.2 | NC_006938.1 | + | 33908 | 0.74 | 0.178233 |
Target: 5'- gGCUcGUCGAGCcagaUCCUCCUCCccauggcuucUGGUGc -3' miRNA: 3'- aCGAcCAGCUCG----AGGAGGAGG----------ACCAC- -5' |
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31715 | 5' | -59.2 | NC_006938.1 | + | 60296 | 1.08 | 0.000503 |
Target: 5'- gUGCUGGUCGAGCUCCUCCUCCUGGUGa -3' miRNA: 3'- -ACGACCAGCUCGAGGAGGAGGACCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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