Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31716 | 3' | -58.2 | NC_006938.1 | + | 5939 | 0.66 | 0.611769 |
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Target: 5'- uGAaCCAGUCCauagaGGaGGGCACCGa-- -3' miRNA: 3'- gCUgGGUCAGGg----CCaCCUGUGGCaac -5' |
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| 31716 | 3' | -58.2 | NC_006938.1 | + | 34260 | 0.66 | 0.601213 |
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Target: 5'- uCGuCUCcGUCCCGGUGGcgucGCACCa--- -3' miRNA: 3'- -GCuGGGuCAGGGCCACC----UGUGGcaac -5' |
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| 31716 | 3' | -58.2 | NC_006938.1 | + | 33814 | 0.67 | 0.56972 |
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Target: 5'- gCGAgCCguGGUCCCGGacaucgugagGGACGCUGUc- -3' miRNA: 3'- -GCUgGG--UCAGGGCCa---------CCUGUGGCAac -5' |
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| 31716 | 3' | -58.2 | NC_006938.1 | + | 50382 | 0.68 | 0.518258 |
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Target: 5'- uGuCCCAcUCCCGGUGG-UGCCGg-- -3' miRNA: 3'- gCuGGGUcAGGGCCACCuGUGGCaac -5' |
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| 31716 | 3' | -58.2 | NC_006938.1 | + | 54157 | 0.69 | 0.459128 |
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Target: 5'- uCGGCCCAGUCCCacGUGGAcCGCa---- -3' miRNA: 3'- -GCUGGGUCAGGGc-CACCU-GUGgcaac -5' |
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| 31716 | 3' | -58.2 | NC_006938.1 | + | 37206 | 0.7 | 0.412714 |
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Target: 5'- aCGGaCCGGUCCCcaguGGUGGACgGCCGg-- -3' miRNA: 3'- -GCUgGGUCAGGG----CCACCUG-UGGCaac -5' |
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| 31716 | 3' | -58.2 | NC_006938.1 | + | 22792 | 0.71 | 0.352847 |
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Target: 5'- aCGGCCUugacaccgucgaGGUCCCGGuUGGGCugCGc-- -3' miRNA: 3'- -GCUGGG------------UCAGGGCC-ACCUGugGCaac -5' |
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| 31716 | 3' | -58.2 | NC_006938.1 | + | 27175 | 0.72 | 0.292287 |
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Target: 5'- ---gCCAGUCCUGGUaGGACACC-UUGg -3' miRNA: 3'- gcugGGUCAGGGCCA-CCUGUGGcAAC- -5' |
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| 31716 | 3' | -58.2 | NC_006938.1 | + | 50289 | 0.79 | 0.103628 |
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Target: 5'- uGACCCGGUCUCGGUagaGGACACCu--- -3' miRNA: 3'- gCUGGGUCAGGGCCA---CCUGUGGcaac -5' |
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| 31716 | 3' | -58.2 | NC_006938.1 | + | 60147 | 1.09 | 0.000776 |
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Target: 5'- uCGACCCAGUCCCGGUGGACACCGUUGg -3' miRNA: 3'- -GCUGGGUCAGGGCCACCUGUGGCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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