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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 31717 | 5' | -54 | NC_006938.1 | + | 63361 | 0.68 | 0.704716 |
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Target: 5'- gCGGcGGCGAGCcgGUCgCCCacgcgGUGGa -3' miRNA: 3'- aGUCuCCGCUCGuaCAG-GGGca---UAUC- -5' |
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| 31717 | 5' | -54 | NC_006938.1 | + | 61411 | 0.67 | 0.74723 |
|
Target: 5'- cUCGGAGGCGuGCAcGUCgCCCc----- -3' miRNA: 3'- -AGUCUCCGCuCGUaCAG-GGGcauauc -5' |
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| 31717 | 5' | -54 | NC_006938.1 | + | 58896 | 1.1 | 0.001348 |
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Target: 5'- gUCAGAGGCGAGCAUGUCCCCGUAUAGa -3' miRNA: 3'- -AGUCUCCGCUCGUACAGGGGCAUAUC- -5' |
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| 31717 | 5' | -54 | NC_006938.1 | + | 45649 | 0.69 | 0.650051 |
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Target: 5'- cUCAGGGGCaGGGCGcagucGUCCUCGccGUAGa -3' miRNA: 3'- -AGUCUCCG-CUCGUa----CAGGGGCa-UAUC- -5' |
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| 31717 | 5' | -54 | NC_006938.1 | + | 39108 | 0.69 | 0.621377 |
|
Target: 5'- cCAGAGGCGGuGCcacgggccgaagcccAUGUCCCaCGUGa-- -3' miRNA: 3'- aGUCUCCGCU-CG---------------UACAGGG-GCAUauc -5' |
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| 31717 | 5' | -54 | NC_006938.1 | + | 26526 | 0.68 | 0.672042 |
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Target: 5'- aCAGGGGcCGGGuCAUGUCCCgCGc---- -3' miRNA: 3'- aGUCUCC-GCUC-GUACAGGG-GCauauc -5' |
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| 31717 | 5' | -54 | NC_006938.1 | + | 25043 | 0.66 | 0.787868 |
|
Target: 5'- uUCAG-GGCG-GCgAUGUCCgCGUAg-- -3' miRNA: 3'- -AGUCuCCGCuCG-UACAGGgGCAUauc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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