Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31719 | 3' | -58.4 | NC_006938.1 | + | 43267 | 0.66 | 0.647826 |
Target: 5'- aCGUCCggaguGCCGUCcCGAUCCGGcGUCUc- -3' miRNA: 3'- -GCAGG-----CGGCGGcGCUAGGUC-UAGAcc -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 19738 | 0.66 | 0.637307 |
Target: 5'- --aCCGCUGCCcgacGCGGUgCGGAaggcgCUGGa -3' miRNA: 3'- gcaGGCGGCGG----CGCUAgGUCUa----GACC- -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 31848 | 0.66 | 0.637307 |
Target: 5'- -uUCCGUCG-CGCGGUCgAGGgugucCUGGa -3' miRNA: 3'- gcAGGCGGCgGCGCUAGgUCUa----GACC- -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 53532 | 0.66 | 0.626783 |
Target: 5'- uGUCCGCCGUCaGCGAgaucgUCCcugugGGAgcgaUGGa -3' miRNA: 3'- gCAGGCGGCGG-CGCU-----AGG-----UCUag--ACC- -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 17318 | 0.66 | 0.626783 |
Target: 5'- -cUCCGCCGUgGCcugcuugaGGUCCAGAg--GGa -3' miRNA: 3'- gcAGGCGGCGgCG--------CUAGGUCUagaCC- -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 32713 | 0.66 | 0.605757 |
Target: 5'- cCGaUCCGCaagcagcucUGCCGCGuggCCAucaagggcacGAUCUGGg -3' miRNA: 3'- -GC-AGGCG---------GCGGCGCua-GGU----------CUAGACC- -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 40642 | 0.66 | 0.605757 |
Target: 5'- uCGUCCGCauCCGCuGGUCCAGcagugccUCUGu -3' miRNA: 3'- -GCAGGCGgcGGCG-CUAGGUCu------AGACc -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 7287 | 0.67 | 0.564015 |
Target: 5'- cCGUCCGCaCGaaGCucUCCGGAUCg-- -3' miRNA: 3'- -GCAGGCG-GCggCGcuAGGUCUAGacc -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 20679 | 0.67 | 0.553687 |
Target: 5'- gCG-CCGCCGCCGUGGUgagaaCCAGGgacaGGc -3' miRNA: 3'- -GCaGGCGGCGGCGCUA-----GGUCUaga-CC- -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 45064 | 0.67 | 0.543417 |
Target: 5'- uCGUCCgGCCGguagaUGCGccgCCAGAUCgGGg -3' miRNA: 3'- -GCAGG-CGGCg----GCGCua-GGUCUAGaCC- -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 60922 | 0.67 | 0.532192 |
Target: 5'- --cUCGUCGCCGUggaggccgcacucGAUCCcgaGGAUCUGGu -3' miRNA: 3'- gcaGGCGGCGGCG-------------CUAGG---UCUAGACC- -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 17645 | 0.68 | 0.523071 |
Target: 5'- -cUCCGCCGCCGaGGcUCC-GAUCaGGa -3' miRNA: 3'- gcAGGCGGCGGCgCU-AGGuCUAGaCC- -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 30807 | 0.68 | 0.513008 |
Target: 5'- cCGUCCGCCuCCuGCGucUCCAGcuccucCUGGa -3' miRNA: 3'- -GCAGGCGGcGG-CGCu-AGGUCua----GACC- -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 33721 | 0.69 | 0.447892 |
Target: 5'- aGUCUcuccuuuggcaccugGCCGUgcagacaCGCGAUCCuguGGAUCUGGu -3' miRNA: 3'- gCAGG---------------CGGCG-------GCGCUAGG---UCUAGACC- -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 23134 | 0.7 | 0.399771 |
Target: 5'- aGUCCGgCGCgGCG-UCgGuGAUCUGGc -3' miRNA: 3'- gCAGGCgGCGgCGCuAGgU-CUAGACC- -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 60039 | 0.7 | 0.399771 |
Target: 5'- cCGgCCuuGCCGCCGUGGUaCCAGGccgcgucgaUCUGGu -3' miRNA: 3'- -GCaGG--CGGCGGCGCUA-GGUCU---------AGACC- -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 56094 | 0.7 | 0.382489 |
Target: 5'- uCGUgCGUCGCCGCGccuAUCUGGAcUCUGa -3' miRNA: 3'- -GCAgGCGGCGGCGC---UAGGUCU-AGACc -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 42350 | 0.7 | 0.374037 |
Target: 5'- aGUCCGCCGCCaCGAUgUCAGccUUGGu -3' miRNA: 3'- gCAGGCGGCGGcGCUA-GGUCuaGACC- -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 51554 | 0.7 | 0.374037 |
Target: 5'- --aCCGCCGCCaucgagggcGCGGUCCGGGUUg-- -3' miRNA: 3'- gcaGGCGGCGG---------CGCUAGGUCUAGacc -5' |
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31719 | 3' | -58.4 | NC_006938.1 | + | 17201 | 0.7 | 0.374037 |
Target: 5'- --aCCGCCGuCCGCGuaGUCC--GUCUGGa -3' miRNA: 3'- gcaGGCGGC-GGCGC--UAGGucUAGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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