Results 1 - 13 of 13 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31719 | 5' | -55.5 | NC_006938.1 | + | 332 | 0.66 | 0.749649 |
Target: 5'- uCUCUCCUaaUGGUUggagagcuGUUGGGCuGGCa -3' miRNA: 3'- cGGGAGGAgcACCAG--------UAACCUGuCCG- -5' |
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31719 | 5' | -55.5 | NC_006938.1 | + | 54743 | 0.66 | 0.73938 |
Target: 5'- cGCCgUCCUUGaGGgCGUUGGcgACAcGGCc -3' miRNA: 3'- -CGGgAGGAGCaCCaGUAACC--UGU-CCG- -5' |
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31719 | 5' | -55.5 | NC_006938.1 | + | 61726 | 0.67 | 0.718538 |
Target: 5'- uCCCUCCUCcUGGaCgg-GGAC-GGCg -3' miRNA: 3'- cGGGAGGAGcACCaGuaaCCUGuCCG- -5' |
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31719 | 5' | -55.5 | NC_006938.1 | + | 49064 | 0.67 | 0.68668 |
Target: 5'- cUCCUCCUCGgagaaGGUCucc-GACAGGa -3' miRNA: 3'- cGGGAGGAGCa----CCAGuaacCUGUCCg -5' |
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31719 | 5' | -55.5 | NC_006938.1 | + | 28139 | 0.67 | 0.68668 |
Target: 5'- aGCCaCUCCUCGUucaCAUUGu-CGGGCa -3' miRNA: 3'- -CGG-GAGGAGCAccaGUAACcuGUCCG- -5' |
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31719 | 5' | -55.5 | NC_006938.1 | + | 20680 | 0.67 | 0.66517 |
Target: 5'- cGCCg-CCgcCGUGGUgAgaaccagGGACAGGCc -3' miRNA: 3'- -CGGgaGGa-GCACCAgUaa-----CCUGUCCG- -5' |
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31719 | 5' | -55.5 | NC_006938.1 | + | 60306 | 0.69 | 0.557344 |
Target: 5'- aGCuCCUCCUCcUGGUgAUUgaaGGGC-GGCa -3' miRNA: 3'- -CG-GGAGGAGcACCAgUAA---CCUGuCCG- -5' |
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31719 | 5' | -55.5 | NC_006938.1 | + | 56748 | 0.7 | 0.536215 |
Target: 5'- aGCCCUcggCCUUGUcGaCcgUGGACAGGUc -3' miRNA: 3'- -CGGGA---GGAGCAcCaGuaACCUGUCCG- -5' |
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31719 | 5' | -55.5 | NC_006938.1 | + | 8065 | 0.71 | 0.455029 |
Target: 5'- gGCCC-CUcCGUGGUCGUcucuUGGcCGGGUg -3' miRNA: 3'- -CGGGaGGaGCACCAGUA----ACCuGUCCG- -5' |
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31719 | 5' | -55.5 | NC_006938.1 | + | 27112 | 0.71 | 0.43577 |
Target: 5'- gGCCacgCCuUCGUGGUgGUgcacGGGCAGGUc -3' miRNA: 3'- -CGGga-GG-AGCACCAgUAa---CCUGUCCG- -5' |
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31719 | 5' | -55.5 | NC_006938.1 | + | 60139 | 0.72 | 0.398712 |
Target: 5'- aGCCCUCCUCGacccaGUCccggUGGACaccguuGGGCg -3' miRNA: 3'- -CGGGAGGAGCac---CAGua--ACCUG------UCCG- -5' |
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31719 | 5' | -55.5 | NC_006938.1 | + | 40431 | 0.73 | 0.338931 |
Target: 5'- aCCCUCUUCcucUGGaUCccUGGACAGGCg -3' miRNA: 3'- cGGGAGGAGc--ACC-AGuaACCUGUCCG- -5' |
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31719 | 5' | -55.5 | NC_006938.1 | + | 55242 | 1.14 | 0.000531 |
Target: 5'- gGCCCUCCUCGUGGUCAUUGGACAGGCa -3' miRNA: 3'- -CGGGAGGAGCACCAGUAACCUGUCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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