Results 41 - 45 of 45 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31720 | 3' | -52.2 | NC_006938.1 | + | 50890 | 0.66 | 0.899903 |
Target: 5'- --cGGCCGAGGcgugcUCAAcgccuuccgcgagGCGGCcgaGGGCCu -3' miRNA: 3'- ucaUCGGCUUC-----AGUU-------------UGCUGa--CCCGG- -5' |
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31720 | 3' | -52.2 | NC_006938.1 | + | 21121 | 0.66 | 0.900598 |
Target: 5'- gGGUuccGGCCGAGGgaguggCAGGCGucGCUccGGCCg -3' miRNA: 3'- -UCA---UCGGCUUCa-----GUUUGC--UGAc-CCGG- -5' |
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31720 | 3' | -52.2 | NC_006938.1 | + | 45661 | 0.66 | 0.900598 |
Target: 5'- --aGGCCgGGAGgccgaGAACGGCUGGaGCg -3' miRNA: 3'- ucaUCGG-CUUCag---UUUGCUGACC-CGg -5' |
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31720 | 3' | -52.2 | NC_006938.1 | + | 54411 | 0.66 | 0.906729 |
Target: 5'- --cGGCCGGAGUCGAggccACcACUGuccucgcGGCCc -3' miRNA: 3'- ucaUCGGCUUCAGUU----UGcUGAC-------CCGG- -5' |
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31720 | 3' | -52.2 | NC_006938.1 | + | 50247 | 0.66 | 0.907397 |
Target: 5'- --aGGCCGggGUC---CGGC-GGGUCc -3' miRNA: 3'- ucaUCGGCuuCAGuuuGCUGaCCCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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