Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31721 | 3' | -54.4 | NC_006938.1 | + | 13341 | 0.66 | 0.784559 |
Target: 5'- gGAUGGCgGUGUcgacaaAGccAGCCgguGCCUGggCg -3' miRNA: 3'- aCUACCGgCACA------UC--UCGG---UGGACaaG- -5' |
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31721 | 3' | -54.4 | NC_006938.1 | + | 8563 | 0.66 | 0.774505 |
Target: 5'- -cAUGGUCaUGgacgAGAGCCGCUcGUUCa -3' miRNA: 3'- acUACCGGcACa---UCUCGGUGGaCAAG- -5' |
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31721 | 3' | -54.4 | NC_006938.1 | + | 6433 | 0.66 | 0.771459 |
Target: 5'- aGGUGGCCGaUGgcaagcacGAGCUcaucgagaccaucuACCUGUUUg -3' miRNA: 3'- aCUACCGGC-ACau------CUCGG--------------UGGACAAG- -5' |
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31721 | 3' | -54.4 | NC_006938.1 | + | 2094 | 0.66 | 0.764302 |
Target: 5'- aGGUGGUCuuUGUGGAGCCGgacagggucacuCCUGUc- -3' miRNA: 3'- aCUACCGGc-ACAUCUCGGU------------GGACAag -5' |
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31721 | 3' | -54.4 | NC_006938.1 | + | 32895 | 0.66 | 0.764302 |
Target: 5'- uUGAacGCgGUGUGGAacucuGCCACCUGa-- -3' miRNA: 3'- -ACUacCGgCACAUCU-----CGGUGGACaag -5' |
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31721 | 3' | -54.4 | NC_006938.1 | + | 39253 | 0.67 | 0.732911 |
Target: 5'- gGAUgcGGCCGUcgagcaccucGUGGAGgCGCUUGUUg -3' miRNA: 3'- aCUA--CCGGCA----------CAUCUCgGUGGACAAg -5' |
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31721 | 3' | -54.4 | NC_006938.1 | + | 29473 | 0.67 | 0.732911 |
Target: 5'- ---cGGCCGUGUAGAGCgCGa-UGUa- -3' miRNA: 3'- acuaCCGGCACAUCUCG-GUggACAag -5' |
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31721 | 3' | -54.4 | NC_006938.1 | + | 11696 | 0.67 | 0.700596 |
Target: 5'- cUGGuUGGCCGUGUuGAGguucuCCACCUcgUCg -3' miRNA: 3'- -ACU-ACCGGCACAuCUC-----GGUGGAcaAG- -5' |
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31721 | 3' | -54.4 | NC_006938.1 | + | 15698 | 0.67 | 0.690769 |
Target: 5'- gGGUcGGCCGUGgucgacugcaggacgGggagcGAGCCGCCUGUg- -3' miRNA: 3'- aCUA-CCGGCACa--------------U-----CUCGGUGGACAag -5' |
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31721 | 3' | -54.4 | NC_006938.1 | + | 14933 | 0.68 | 0.667671 |
Target: 5'- -aGUGGCCGgagGUGGcGCCAUUUGUc- -3' miRNA: 3'- acUACCGGCa--CAUCuCGGUGGACAag -5' |
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31721 | 3' | -54.4 | NC_006938.1 | + | 30010 | 0.68 | 0.63444 |
Target: 5'- aGGUGGCCGUGgcagcucauGAGCCagGCCUc--- -3' miRNA: 3'- aCUACCGGCACau-------CUCGG--UGGAcaag -5' |
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31721 | 3' | -54.4 | NC_006938.1 | + | 17186 | 0.69 | 0.579138 |
Target: 5'- gUGAUGGUCGgcaugGUGG-GCCAgCUGgUCu -3' miRNA: 3'- -ACUACCGGCa----CAUCuCGGUgGACaAG- -5' |
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31721 | 3' | -54.4 | NC_006938.1 | + | 14001 | 0.7 | 0.546418 |
Target: 5'- gGAUGGCC-UGUAugcuGCCACC-GUUCu -3' miRNA: 3'- aCUACCGGcACAUcu--CGGUGGaCAAG- -5' |
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31721 | 3' | -54.4 | NC_006938.1 | + | 4203 | 0.78 | 0.194355 |
Target: 5'- cGAUGGUCGUGccAGAGCCACCUc--- -3' miRNA: 3'- aCUACCGGCACa-UCUCGGUGGAcaag -5' |
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31721 | 3' | -54.4 | NC_006938.1 | + | 54959 | 1.11 | 0.000977 |
Target: 5'- gUGAUGGCCGUGUAGAGCCACCUGUUCg -3' miRNA: 3'- -ACUACCGGCACAUCUCGGUGGACAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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