Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31722 | 5' | -57.2 | NC_006938.1 | + | 24482 | 0.66 | 0.629018 |
Target: 5'- --gGGGCCGAagagccAGGUCUCGUacaUGCCa- -3' miRNA: 3'- cuaCCUGGCUa-----UCCAGAGCG---GCGGcu -5' |
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31722 | 5' | -57.2 | NC_006938.1 | + | 12791 | 0.67 | 0.607589 |
Target: 5'- --aGGACgGAgcGGagCUCGUCGCCGc -3' miRNA: 3'- cuaCCUGgCUauCCa-GAGCGGCGGCu -5' |
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31722 | 5' | -57.2 | NC_006938.1 | + | 40117 | 0.67 | 0.596901 |
Target: 5'- --cGGacagcGCCGGUcagccGGGUgUCGCgGCCGAg -3' miRNA: 3'- cuaCC-----UGGCUA-----UCCAgAGCGgCGGCU- -5' |
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31722 | 5' | -57.2 | NC_006938.1 | + | 56767 | 0.67 | 0.596901 |
Target: 5'- cGUGGACaGGUcggccuuGGcCUCGCCGCCa- -3' miRNA: 3'- cUACCUGgCUAu------CCaGAGCGGCGGcu -5' |
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31722 | 5' | -57.2 | NC_006938.1 | + | 17636 | 0.67 | 0.586242 |
Target: 5'- -cUGGGCCG-----UCUcCGCCGCCGAg -3' miRNA: 3'- cuACCUGGCuauccAGA-GCGGCGGCU- -5' |
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31722 | 5' | -57.2 | NC_006938.1 | + | 30843 | 0.67 | 0.575618 |
Target: 5'- --aGGucGCCGA--GGUCUCuggggcacugGCCGCCGAc -3' miRNA: 3'- cuaCC--UGGCUauCCAGAG----------CGGCGGCU- -5' |
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31722 | 5' | -57.2 | NC_006938.1 | + | 58688 | 0.68 | 0.533638 |
Target: 5'- --cGGAUUGAaGGGcUUCGCCGUCGGa -3' miRNA: 3'- cuaCCUGGCUaUCCaGAGCGGCGGCU- -5' |
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31722 | 5' | -57.2 | NC_006938.1 | + | 203 | 0.68 | 0.533638 |
Target: 5'- cGUGGG-CGAccGG-CUCGCCGCCGc -3' miRNA: 3'- cUACCUgGCUauCCaGAGCGGCGGCu -5' |
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31722 | 5' | -57.2 | NC_006938.1 | + | 24535 | 0.73 | 0.286274 |
Target: 5'- cGUGGAUCGugagacAGGUC-CGCCGCCGc -3' miRNA: 3'- cUACCUGGCua----UCCAGaGCGGCGGCu -5' |
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31722 | 5' | -57.2 | NC_006938.1 | + | 30016 | 0.8 | 0.099766 |
Target: 5'- --cGGACgCGGUAucuGGUCUCGUCGCCGAc -3' miRNA: 3'- cuaCCUG-GCUAU---CCAGAGCGGCGGCU- -5' |
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31722 | 5' | -57.2 | NC_006938.1 | + | 54209 | 1.08 | 0.000908 |
Target: 5'- uGAUGGACCGAUAGGUCUCGCCGCCGAa -3' miRNA: 3'- -CUACCUGGCUAUCCAGAGCGGCGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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