Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31724 | 3' | -58.7 | NC_006938.1 | + | 33797 | 0.66 | 0.571638 |
Target: 5'- gGAUGgcugCCAUGUcguagcuGCUCCUCGGCgGUc- -3' miRNA: 3'- -UUGCa---GGUGCA-------CGAGGAGCCGgCAcu -5' |
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31724 | 3' | -58.7 | NC_006938.1 | + | 37893 | 0.66 | 0.562158 |
Target: 5'- --aGUCC-CGUGuCUCgCUCGG-CGUGAc -3' miRNA: 3'- uugCAGGuGCAC-GAG-GAGCCgGCACU- -5' |
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31724 | 3' | -58.7 | NC_006938.1 | + | 4225 | 0.66 | 0.552721 |
Target: 5'- -uCGUCaACGUGCUCCgccucuaccagaucaCGGCCG-GAu -3' miRNA: 3'- uuGCAGgUGCACGAGGa--------------GCCGGCaCU- -5' |
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31724 | 3' | -58.7 | NC_006938.1 | + | 12675 | 0.67 | 0.470484 |
Target: 5'- aGGCGUUgagCACGa---CCUCGGCCGUGAu -3' miRNA: 3'- -UUGCAG---GUGCacgaGGAGCCGGCACU- -5' |
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31724 | 3' | -58.7 | NC_006938.1 | + | 21328 | 0.68 | 0.451125 |
Target: 5'- gAACuuggCCACGguggacUGCUCCUCGGUCGUc- -3' miRNA: 3'- -UUGca--GGUGC------ACGAGGAGCCGGCAcu -5' |
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31724 | 3' | -58.7 | NC_006938.1 | + | 39232 | 0.68 | 0.432211 |
Target: 5'- gGACGaucUCCACGUcCUCCaggaugCGGCCGUcGAg -3' miRNA: 3'- -UUGC---AGGUGCAcGAGGa-----GCCGGCA-CU- -5' |
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31724 | 3' | -58.7 | NC_006938.1 | + | 25549 | 0.7 | 0.355666 |
Target: 5'- cGCGUCCcuugagGCGcaggauguugcccUGCUCCgugacguuccaacccUCGGCCGUGAa -3' miRNA: 3'- uUGCAGG------UGC-------------ACGAGG---------------AGCCGGCACU- -5' |
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31724 | 3' | -58.7 | NC_006938.1 | + | 59296 | 0.72 | 0.24185 |
Target: 5'- -cCGUUCACGUcCUCCUggaacggcagguaucCGGCCGUGAu -3' miRNA: 3'- uuGCAGGUGCAcGAGGA---------------GCCGGCACU- -5' |
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31724 | 3' | -58.7 | NC_006938.1 | + | 58073 | 0.74 | 0.175589 |
Target: 5'- cGCGUCCugGUGgUCCUCGG-CGUa- -3' miRNA: 3'- uUGCAGGugCACgAGGAGCCgGCAcu -5' |
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31724 | 3' | -58.7 | NC_006938.1 | + | 36106 | 0.74 | 0.175589 |
Target: 5'- ---cUCCuCGUGCUCCUCGGCCGc-- -3' miRNA: 3'- uugcAGGuGCACGAGGAGCCGGCacu -5' |
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31724 | 3' | -58.7 | NC_006938.1 | + | 53367 | 1.06 | 0.000892 |
Target: 5'- gAACGUCCACGUGCUCCUCGGCCGUGAc -3' miRNA: 3'- -UUGCAGGUGCACGAGGAGCCGGCACU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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